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Stereoselective biocatalysis in green solvents. European Biotechnology Congress

The introduction of green chemistry principles into the preparation of various commercial products, such as chiral building blocks for drug preparation, is becoming an imperative in the scientific community. There are numerous tools of green chemistry such as the use of alternative solvents and environmentally benign raw (or renewable) materials and performing reactions by (bio)catalysis. The aim of this study is the application of two biocatalysts, purified lipase enzymes and whole-cell biocatalysis (plant cells), for the preparation of interesting chiral intermediates within environmentally friendly solvents (natural deep eutectic solvents, NADESs). For that purpose, enantioselective hydrolysis of (R, S)-1- phenylethyl acetate by immobilized Candida antarctica lipase B and Daucus carota root in cholinium-based NADESs as solvents, was conducted. Based on the results, we can conclude that for both biocatalysts the NADESs are superior to the traditional solvents when used in enantioselective hydrolysis of (R, S)-1-phenylethyl acetate, in terms of product yield and the enantiomeric excess (e.e.). Finally, if we consider environmental benefits of applying NADESs as solvents, they are excellent candidates for environment-friendly biocatalysis.

biocatalysis, Daucus carota, enantioselectivity, lipase, natural deep eutectic solvents, (R, S)-1-phenylethyl acetate

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