engleski

Histomorphological and histochemical analysis of some skeletal muscles in fattened bulls

White fast-twitch glycolityc (FG) fibres of large diameter, red slow-twitch oxidative (SO) fibres of small diameter and intermediate fast-twitch oxidative (FOG) glycolityc fibres of intermediate diameter were found in the samples of m. obliquus abdominis externus, m. rectus abdominis, m. triceps brachii (caput longum), m. pectoralis superficialis, m. longissimus dorsi (2 samples), m. psoas major and m. semimembranaceus taken from both body sides of 5 fattened bulls, 15 months old and weighing 400 kg. The white fibres dominate in all muscle samples and they show a strong activity of standard (S ATP) and alkaline stable adenosine triphosphatase (AL ATP), a weak activity of lactate and succinate dehydrogenase (LDH and SDH) and of acid stable adenosine triphosphatase (AC ATP), a low content of lipids and a high content of glycogen. In 4 muscles the red fibres are more numerious than the intermediate ones. They show a strong activity of LDH and SDH, of AC ATP, a weak activity of S ATP and AL ATP, a high content of lipids and a low content of glycogen. The FOG fibres are more numerious that the SO ones in 3 muscles (4 samples), and they show a strong activity of S ATP and AL ATP, a weak activity of AC ATP, a moderate activity of LDH and SDH as well as a varying content of lipids and glycogen. The muscle fibre diameters ranged from 20 to 115 micrometers, but the largest average diameter was found in m. psoas major, the smallest being in m. obliquus abdominis externus. The difference in muscle fibre diameters between the left and the right side of the body were not significant (P>0, 05).

muscle fibre diameter; histomorphological analysis; oxidative enzymes; myosin adenosine triphosphatase; fattened bulls

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