engleski

Fluorophore one-atom-controlled recognition of DNA/RNA secondary structures

Cyanine dyes are commonly used as molecular probes for nucleic acids and proteins due to well known fluorescence “switch-on” upon binding to target, while nonfluorescent in free form. Here we present two novel cyanine-amino acid conjugates differing only in a halogen substituent, prepared by Cu-catalyzed azide- alkyne cycloaddition. Remarkable difference between fluorine- and chlorine- analogue interactions with polynucleotides was documented by circular dichroism spectroscopy and additionally analysed by molecular modeling simulations. Biological experiments in the cells showed that studied conjugates are not localised in nucleus as parent analogous cyanine dye (TO), but target mitochondria.

DNA/RNA recognition• mitochondrial dyes • groove binding • induced circular dichroism

Rad je kao predavanje prezentiran i na skupovima: - 25th Croatian meeting of chemists and chemical engineers, održanom od 9.-22.04.2017.g., Poreč, Hrvatska ; uz domaću recenziju objavljen kao sažetak, te na skupu 4th EFMC Young Medicinal Chemist Symposium, održanom od 31.08.-01.09.2017.g. Beč, Austrija.