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Infection and Colonization of Nicotiana benthamiana by Grapevine leafroll-associated virus 3 (CROSBI ID 241111)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Prator, Cecilia A. ; Kashiwagi, Chloe M. ; Vončina, Darko ; Almeida, Rodrigo P.P. Infection and Colonization of Nicotiana benthamiana by Grapevine leafroll-associated virus 3 // Virology (New York, N.Y.), 510 (2017), 60-66. doi: 10.1016/j.virol.2017.07.003

Podaci o odgovornosti

Prator, Cecilia A. ; Kashiwagi, Chloe M. ; Vončina, Darko ; Almeida, Rodrigo P.P.

engleski

Infection and Colonization of Nicotiana benthamiana by Grapevine leafroll-associated virus 3

Grapevine leafroll disease is an increasing problem in all grape-growing regions of the world. The most widespread agent of the disease, Grapevine leafroll-associated virus 3 (GLRaV-3), has never been shown to infect species outside of the genus Vitis. Virus transmission to several plant species used as model systems was tested using the vine mealybug, Planococcus ficus. We show that GLRaV-3 is able to infect Nicotiana benthamiana. Working with GLRaV-3 infected N. benthamiana revealed distinct advantages in comparison with its natural host Vitis vinifera, yielding both higher viral protein and virion concentrations in western blot and transmission electron microscopy observations, respectively. Immunogold labelling of thin sections through N. benthamiana petioles revealed filamentous particles in the phloem cells of GLRaV-3 positive plants. Comparison of assembled whole genomes from GLRaV-3 infected V. vinifera vs. N. benthamiana revealed substitutions in the 5′ UTR. These results open new avenues and opportunities for GLRaV-3 research.

Grapevine leafroll associated virus 3, Grapevine leafroll disease, Closteroviridae, Ampelovirus, Nicotiana benthamiana

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Podaci o izdanju

510

2017.

60-66

objavljeno

0042-6822

10.1016/j.virol.2017.07.003

Povezanost rada

Poljoprivreda (agronomija), Biologija

Poveznice
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