engleski

INTERLEUKINE-8 POLYMORPHISM AND PROSTATE CANCER SUSCEPTIBILITY IN EASTERN CROATIAN POPULATION

BACKGROUND-AIM Angiogenesis plays an important role in the development, growth and spread of solid tumors, including prostate cancer. Numerous studies link potentially functional single nucleotide polymorphisms (SNP) in genes important in prostate angiogenesis with increased risk and aggressive form of prostate cancer. The aim of our study was to investigate the influence of polymorphism in the pro- angiogenic IL-8 -251 (rs4073) cytokine genes with the prostate cancer risk and aggressiveness in eastern Croatian patients. METHODS A total of 275 subjects from eastern Croatia were included in the study, 120 prostate cancer patients (CaP), 120 benign prostate hyperplasia (BHP) patients and 35 controls. They were genotyped for IL-8 -251 (rs4073) polymorphism using real-time PCR (LightCycler Instrument, Roche Diagnostics) and melting curve analysis method. All groups were agematched (mean age 67.5 years). CaP patients were classified in two groups according to Gleason score (≤7 and >7). χ2 test was used to compare distribution of IL-8 polymorphism genotypes. Relative risk was estimated by the Odds ratio (OR). RESULTS There was no significant statistical difference in the frequency of genotypes between controls and patients with CaP and BHP (χ2=1.158, P=0.885, OR=0.862, 95%CI=0.295- 2.524). An increased frequency of IL-8 -251 AA genotype is noted in CaP patients with the Gleason score >7 (25% vs 16.7%), although with no statistical significance (χ2=1.569, P=0.456). There was no significant statistical difference in the frequency of genotypes and the presence of metastatic disease in CaP patients (χ2=2.057, P=0.3576, OR=0.933, 95%CI=0.424-2.056). CONCLUSION Our data suggest that, although, there is a tendency in increased frequency of IL-8 -251 AA genotype in CaP patients according to Gleason score, IL-8 -251 SNP-s is not associated with prostate cancer development, progression and/or presence of distant metastasis in eastern Croatian population. The limiting factor of this study was a relatively small number of patients ; therefore we suggest that a larger group might show association of AA genotype with the risk of developing more aggressive forms of prostate cancer in the population of eastern Croatian population.

Interleukin-8, polymorphism, prostata cancer

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