engleski

Association of IL-1 ssociation of IL-1band IL- 10 Polymorphisms and IL-10 Polymorphisms with Prostate Cancer Risk and Grade of Disease ith Prostate Cancer Risk and Grade of Disease in Eastern Croatian Population

Single nucleotide polymorphisms (SNPs) in the promotor regions of cytokine genes included in angiogenesis may infl uence prostate cancer (PCa) development via regulation of the pathways of tumor angiogenesis. The aim of the present study was to investigate the association of IL- 1b+3954 (rs1143634) and IL-10-1082 (rs1800896) polymorphisms with PCa risk and aggressiveness in eastern Croatian patients. One hundred twenty PCa patients and 120 benign prostatic hyperplasia (BPH) controls were genotyped using real-time PCR (LightCycler Instrument, Roche Diagnostics) and the melting curve analysis method. There was no signifi cant difference in the frequency of genotypes for the two polymorphisms between PCa patients and controls (χ2=0.857, p=0.355 for IL-b1 ; χ2=0.026, p=0.872 for IL-10). Carriers of the IL-10-1082A>G variant were found to be associated with the Gleason score (GS)>7 (AA versus GA+GG, OR=3.47, 95% CI 1.11–10.88, p=0.033). There was no signifi cant difference in the frequency of genotypes for the two polymorphisms and the presence of metastatic disease in PCa patients. These results suggest that tested SNPs associated with differential production of IL-1b and IL-10 are not risk factors for PCa and do not correlate with the presence of distant metastasis in eastern Croatians. We found that IL-10-1082 GA+/or GG carriers have a higher risk of developing PCa with GS>7 in eastern Croatians.

prostate cancer, benign prostatic hyperplasia, Gleason score, angiogenesis, SNP, interleukin-1b, interleukin-10, eastern Croatia, risk, grade

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