engleski

Evaluation of molecular mechanism differences in in vitro models of drug induced and fatty- acids induced NAFLD

Throughout the last decades, prevalence of NAFLD (non-alcoholic fatty liver disease) is dramatically increasing. NAFLD includes a spectrum of disorders such as steatosis, non- alcoholic steatohepatitis and fibrosis/cirrhosis. It is estimated that approximately 20% of adult population has hepatic steatosis and 2-3% suffer from non- alcoholic steatohepatitis. Previously, numerous drugs had been proven to cause fatty liver disease, however molecular mechanisms of drug induced NAFLD are not fully elucidated. Therefore, the aim of our study was to evaluate molecular mechanisms involved in development of drug-induced fatty liver disease and fatty-acid induced NAFLD in vitro. FAs and hepatotoxic drugs had lipogenic effect on triglyceride accumulation and steatogenesis in hepatocyte cell cultures. This steatogenic effect was characterized by differences in excessive accumulation of trilycerides together with the appearance of Oil Red O–stained lipid vesicles and overexpression of genes involved in lipogenesis and droplet formation.

NAFLD ; cell culture ; fatty acids

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