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Allele Frequencies and Genetic Parameters for 15 STR Loci in the Population of Bosnia and Herzegovina

Allele frequencies of the 15 polymorphic STR loci (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX, FGA) for Bosnian and Herzegovinian population were established and statistical forensic parameters were calculated. In order to expand national population data with allele frequencies and statistical data for fifteen STR loci, 1000 unrelated individuals born in Bosnia and Herzegovina volontarly participate in the study. Qiagen DaeasyTM Tissue Kit was used for DNA extraction from buccal swabs. Genomic DNA amplification was performed using PowerPlex® 16 System which enables amplification and detection of 15 STR and amelogenin. For PCR amplification GeneAmp PCR System 9700 (Applied Biosystems) was used. The capillary electrophoresis of amplified products was carried in an ABI 310 Genetic Analyzer while numerical allele designations were determined using GeneMapper®ID software v.3.2. Microsoft Excel workbook template—PowerStats was used for calculating allele frequencies, matching probability (MP), power of discrimination (PD), power of exclusion (PE) and typical paternity index (PI). Powermarker v.3.25 was used for calculation of number of alleles (AN), deviation from Hardy–Weinberg equilibrium, observed and expected heterozygosity (Ho and He) and polymorphism information content (PIC). Exact test of population differentiation was estimated using Arlequin v.3.5.1.2. After Bonferroni’s correction, statistical significance for deviation from Hardy– Weinberg equilibrium was considered as P<0.01, while for population differentiation test P<0.001. Number of effective alleles (AE) was estimated by 1/∑pi2, where p is allele frequency for particular locus. Ratio of effective and detected allele numbers and its statistical significance were also calculated. No statistically significant deviation (P>0.05) from HWE was found for analyzed loci, except for D8S1179 locus, which was not significant after applying the Bonferroni’s correction (P>0.01). Heterozygosity excess has been detected for D3S1358, D21S11, D18S51, D16S539, vWA, TPOX loci. Total of 160 alleles were detected, among which 32 are considered as rare alleles (frequency <0.005). The highest number of alleles was detected for PentaE (18) and the lowest for TH01 (7).

STR markers, allele frequencies, forensic parameters, DNA database B&H population

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