The dynamic of Tff1 and Tff3 peptide presence in mouse embryonic gastrointestinal system from day 14 to 18 of intrauterine development (CROSBI ID 650118)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Bijelić, Nikola ; Belovari, Tatjana ; Lovrić Ivana ; Baus Lončar, Mirela
engleski
The dynamic of Tff1 and Tff3 peptide presence in mouse embryonic gastrointestinal system from day 14 to 18 of intrauterine development
INTRODUCTION: Trefoil factor family (Tff) peptides are important for protection and restitution of gastrointestinal (GI) mucosa. They are found in a large part of the GI tract, from the oral cavity to the rectum, as well as in some glands associated with the GI tract. Endodermal to gastrointestinal epithelium differentiation in mice occurs during 15th or 16th day of intrauterine development. Tff peptides have been researched in this context to an extent, but no systemic immunohistochemical studies have been performed in order to describe the presence of these peptides depending on the developmental stage. The aim of this research was to describe the presence of Tff1 and Tff3 peptide in the GI mucosa from day 14 to 18 of mouse intrauterine development. MATERIALS AND METHODS: 4% paraformaldehyde fixed and paraffin-embedded CD1 mouse embryos at gestational days 14 to 18 were used. Sagittal sections 6μm thick were cut and transferred to adhesive slides. The embryos were stained with purified proprietary, self-made anti-Tff1 and anti-Tff3 rabbit primary polyclonal antibodies. Labeled Streptavidin Biotin (LSAB) method was used, with PBS as a negative control. Mayer’s hematoxylin was used for counterstaining. RESULTS: The localization of Tff1 and Tff3 peptide was similar, but somewhat different from that of adult mice, with specific localization patterns for both peptides. Immunohistochemistry showed weak or no signal at 14-day stage and strenghthening of the signal until day 18. Both peptides were found in stomach, small intestine, colon, liver and pancreas. Their localization and distribution depended on the developmental stage. CONCLUSION: Tff1 and Tff3 peptides can be viewed as markers of the GI mucosa maturation during embryonic development. They also might have an influence on the differentiation of endoderm into mature epithelium. Further research might confirm or deny that, as well as show the extent of their influence on embryonic development of GI system.
Trefoil Factor ; Mouse ; Embryonic Development ; Immunohistochemistry
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Podaci o prilogu
91-92.
2017.
objavljeno
Podaci o matičnoj publikaciji
17th International European Light Microscopy Initiative Meeting : Program and Abstract Book
Weber, Igor ; Tolić, Iva ; Kovačević, Goran ; Vidoš, Ana
Zagreb:
978-953-7941-16-1
Podaci o skupu
17th International European Light Microscopy Initiative Meeting (ELMI)
poster
23.05.2017-26.05.2017
Dubrovnik, Hrvatska