engleski

Dihydrochalcones in old apple varieties from Croatia

Dihydrochalcones are the only subgroup of polyphenols that is specific to apples. They showed many beneficial bioactivities. In this work, the aim was to analyze dihydrochalcones in old apple varieties from Croatia to see if varieties differ amongst themselves according to dihydrochalcone percentage. Dihydrochalcones were extracted from the peel and flesh of five old apple varieties (Lještarka, Ljetna rebrača, Slavonska srčika, Zimnjara, Adamova zvijezda) and from wild apples (crabapples). Reverse-phase high performance liquid chromatography was used to identify dihydrochalcones (phloretin-2’-xyloglucoside and phloretin-2’-glucoside in the flesh ; phloretin-2’-glucoside in the peel) and to express their percentage in relation to all other polyphenol subclasses. Dihydrochalcones occupied from 3 to 12.4 % in the flesh and from 0.8 to 10.8 % in the peel. Slavonska srčika and Adamova zvijezda showed higher dihydrochalcone percent in the flesh and peel. Principal component analysis also confirmed that Slavonska srčika and Adamova zvijezda differed from other varieties by higher dihydrochalcone percent. The pattern in the dihydrochalcone distribution was not shown. Although dihydrochalcones do not occupy a high portion in the polyphenol content, they are still an important feature of old varieties.

phloretin ; phloridzin ; dihydrochalcones ; old varieties ; apple

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