engleski

Recovery of Highly Fragmented nDNA from Skeletal Material for SNP-based MPS Analysis

Molecular characterization and identification of skeletal remains continues to be a focus of anthropologists and forensic scientists. Due to the highly fragmented nature of the DNA recovered from older skeletal material, single nucleotide polymorphism (SNP) analysis may be desirable as the targets are typically 40 to 70 bps in length. When scores of SNPs are typed simultaneously they can be used for identity, and can provide phenotypic and ancestry information. Current methods of DNA extraction in forensics have been optimized for recovery of larger fragment sizes suitable for STRs. For SNP typing it would be desirable to use methods optimized for recovery of the relatively abundant smaller fragments. This study aims to identify a scalable protocol for the recovery of DNA under 150 bp in length and demonstrate its success using the Promega 10-plex mtDNA kit and the Illumina ForenSeq system of STR and SNP loci.

nDNA ; skeletal material ; MPS Analysis

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