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Pregled bibliografske jedinice broj: 882264

Late Endosomal Recycling of Open MHC-I Conformers


Mahmutefendić, Hana; Blagojević Zagorac, Gordana; Grabušić, Kristina; Karleuša, Ljerka; Maćešić, Senka; Momburg, Frank; Lučin, Pero
Late Endosomal Recycling of Open MHC-I Conformers // Journal of cellular physiology, 232 (2017), 4; 872-887 doi:10.1002/jcp.25495 (međunarodna recenzija, članak, znanstveni)


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Naslov
Late Endosomal Recycling of Open MHC-I Conformers

Autori
Mahmutefendić, Hana ; Blagojević Zagorac, Gordana ; Grabušić, Kristina ; Karleuša, Ljerka ; Maćešić, Senka ; Momburg, Frank ; Lučin, Pero

Izvornik
Journal of cellular physiology (0021-9541) 232 (2017), 4; 872-887

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Major Histocompatibility Class I ; Open MHC-I conformers ; Endosomal trafficking ; Endosomal recycling ; Late endosomes

Sažetak
With an increasing number of endosomal cargo molecules studied, it is becoming clear that endocytic routes are diverse, and the cell uses more pathways to adjust expression of cell surface proteins. Intracellular itinerary of integral membrane proteins that avoid the early endosomal recycling route is not enough studied. Therefore, we studied endocytic trafficking of empty Ld (eLd ) molecules, an open form of murine MHC-I allele, in fibroblast-like cells. Pulse labeling of cell surface eLd with mAbs and internalization kinetics suggest two steps of endosomal recycling: rapid and late. The same kinetics was also observed for human open MHC-I conformers. Kinetic modeling, using in-house developed software for multicompartment analysis, colocalization studies and established protocols for enriched labeling of the late endosomal (LE) pool of eLd demonstrated that the late step of recycling occurs from an LE compartment. Although the majority of eLd distributed into pre-degradative multivesicular bodies (MVBs), these LE subsets were not a source for eLd recycling. The LE recycling of eLd did not require Rab7 membrane domains, as demonstrated by Rab7-silencing, but required vectorial LE motility, suggesting that LE recycling occurs from dynamic tubulovesicular LE domains prior segregation of eLd in MVBs. Thus, our study indicates that LE system should not be simply considered as a feeder for loading of the degradative tract of the cell but also as a feeder for loading of the plasma membrane and thereby contribute to the maintenance of homeostasis of plasma membrane proteins.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-IP-2014-09-9564 - Karakterizacija preuređivanja endosomalnih membranatijekom rane faze infekcije mišjim citomegalovirusom koje vodi k formiranju kompleksa za stvaranje viriona (AsCo) (Lučin, Pero, HRZZ - 2014-09) ( POIROT)

Ustanove:
Medicinski fakultet, Rijeka,
Građevinski fakultet, Rijeka

Citiraj ovu publikaciju

Mahmutefendić, Hana; Blagojević Zagorac, Gordana; Grabušić, Kristina; Karleuša, Ljerka; Maćešić, Senka; Momburg, Frank; Lučin, Pero
Late Endosomal Recycling of Open MHC-I Conformers // Journal of cellular physiology, 232 (2017), 4; 872-887 doi:10.1002/jcp.25495 (međunarodna recenzija, članak, znanstveni)
Mahmutefendić, H., Blagojević Zagorac, G., Grabušić, K., Karleuša, L., Maćešić, S., Momburg, F. & Lučin, P. (2017) Late Endosomal Recycling of Open MHC-I Conformers. Journal of cellular physiology, 232 (4), 872-887 doi:10.1002/jcp.25495.
@article{article, year = {2017}, pages = {872-887}, DOI = {10.1002/jcp.25495}, keywords = {Major Histocompatibility Class I, Open MHC-I conformers, Endosomal trafficking, Endosomal recycling, Late endosomes}, journal = {Journal of cellular physiology}, doi = {10.1002/jcp.25495}, volume = {232}, number = {4}, issn = {0021-9541}, title = {Late Endosomal Recycling of Open MHC-I Conformers}, keyword = {Major Histocompatibility Class I, Open MHC-I conformers, Endosomal trafficking, Endosomal recycling, Late endosomes} }
@article{article, year = {2017}, pages = {872-887}, DOI = {10.1002/jcp.25495}, keywords = {Major Histocompatibility Class I, Open MHC-I conformers, Endosomal trafficking, Endosomal recycling, Late endosomes}, journal = {Journal of cellular physiology}, doi = {10.1002/jcp.25495}, volume = {232}, number = {4}, issn = {0021-9541}, title = {Late Endosomal Recycling of Open MHC-I Conformers}, keyword = {Major Histocompatibility Class I, Open MHC-I conformers, Endosomal trafficking, Endosomal recycling, Late endosomes} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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