engleski

Expression of bone morphogenetic proteins in colorectal cancer

Bone morphogenetic proteins (BMPs) are a group of growth factors primarily isolated from bone according to their osteoinductive ability. The pleotropic nature of BMPs leads them to being called the body morphogenetic proteins. BMPs are included in various processes like cellular migration, proliferation and differentiation and as TGFbeta family members in epithelial- mesenchymal transition. In that way BMPs influence many physiological and pathological conditions. The aim of this study was to investigate the expression of BMPs in colorectal cancer (CRC) samples of patients who underwent the CRC surgery. The expression of BMPs was examined in frequently diagnosed stages of CRC (Dukes’B and Dukes’C stage) by RT-qPCR . Furthermore or also, we have analyzed BMP7 expression in CRC samples by immunohistochemistry using a polyclonal anti- BMP7 antibody. The clinicopathologic data such as age, gender, Dukes’ staging and differentiation grade are also analyzed. Our results showed that colorectal cancer occurred most frequently in men (75%) and in elderly people (more than 60 years ; 85%). The most common site of primary carcinoma was sigmoid colon and rectum (75%) and moderately differentiated (55%). In both stages of CRC, we found the downregulation of BMP4 and BMP7 expression in comparison to the normal colon samples which is significantly higher in Dukes’B stage for BMP4 and in Dukes’C stage for BMP7. The expression of BMP6 was slightly upregulated and showed no statistically significant difference in these stages of CRC. BMP7 expression determined by immunohistochemistry decreased also progressively by higher tumor stage. These results suggest that BMPs beside their importance in bone metabolism have a prominent role in the carcenogenesis and the alterations of their expression could be associated with cancer aggressiveness and progression.

bone morphogenetic proteins, colorectal cancer

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