Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces (CROSBI ID 240154)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Pigac, Jasenka ; Vujaklija, Dušica ; Toman, Zora ; Gamulin, Vera ; Schrempf, Hildgund Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces // Plasmid, 19 (1988), 3; 222-230. doi: 10.1016/0147-619X(88)90040-6

Podaci o odgovornosti

Pigac, Jasenka ; Vujaklija, Dušica ; Toman, Zora ; Gamulin, Vera ; Schrempf, Hildgund

engleski

Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces

Structural instability of a hybrid plasmid pZG1, consisting of Escherichia coli pBR322 and Streptomyces pIJ350 plasmids, has been studied in Streptomyces. After transformation of S. lividans 1326 and S. rimosus R6 protoplasts with pZG1, transformants harbored the intact pZG1 and various deleted plasmid forms. The pattern of deleted plasmids varied with the transformant colony age and changed upon subcultivation. The presence of intact pZG1 in at least a part of the Streptomyces colonies indicated that the plasmid was capable of replicating in the transformants and that deletion events occurred after at least one round of replication. Less instability of pZG1 in S. rimosus R6 was observed when this strain was transformed with the DNA isolated from the same strain. pZG1 and its various derivatives were found in S. lividans 1326 and S. rimosus R6 as double- and single-stranded DNA molecules. Structural instability of pZG1 could therefore be due at least in part to the presence of single- stranded DNA.

structural instability ; bifunctional plasmid

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

19 (3)

1988.

222-230

objavljeno

0147-619X

1095-9890

10.1016/0147-619X(88)90040-6

Povezanost rada

Biologija

Poveznice
Indeksiranost