Octopus vulgaris immune response at gene expression level by in vitro immunostimulation of hemocytes (CROSBI ID 649081)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Petrić, Mirela ; Baldascino, Elena ; Lauritano, Chiara ; Zarrella, Ilaria ; Ponte, Giovanna ; Fiorito, Graziano
engleski
Octopus vulgaris immune response at gene expression level by in vitro immunostimulation of hemocytes
Investigations of the cephalopod immune system have recently become of great importance due to the increasing interest for aquaculture, for the role of potential pathogens on the quality of the “sea-products” for human consumption and for concern about cephalopod welfare since the inclusion of this taxon in the Directive 2010/63/EU on the protection of animals utilized for scientific purposes. Cephalopods rely solely on innate immunity and hemocytes are suggested to play a key role in the immune-system response to challenges (e.g. through phagocytosis, encapsulation, infiltration of pathogens). Aim of this research was to study the elements of cephalopod immune response at gene expression level by in vitro immunostimulation of Octopus vulgaris hemocytes. Hemolymph cell suspension was plated with Squid Ringer’s Solution and kept at 18 °C prior immune-stimulation with commercially available immunostimulants: LPS (Escherichia coli lipopolysaccharides) as bacterial imitator, Poly I:C (polyinosinic-polycytidylic acid sodium salt) as viral imitator and zymosan, a yeast cell extract eliciting fungal type infection response. As control, hemocytes were kept under the same conditions as the immunostimulated ones. Immunostimulation was accomplished within different time- points: 1, 4, 24 and 48 hours. At the end of each time-point hemolymph suspension was collected and centrifuged following pellet resuspension in TriReagent. Total RNA was extracted from hemocytes following manufacturer’s protocol (InVitrogen) and RNA samples were retrotranscripted into cDNA, which was utilized as template for PCR and RT-qPCR analyses. The expression levels of specific gene of interest, immune-related genes and genes associated to the antioxidant system, stress and detoxification, was analyzed. In general, for all the treatments (LPS, Poly I:C and zymosan) and the time points (1, 4, 24 and 48 h) the majority of investigated antioxidant genes (sod, gpx and gsh-s) were up-regulated after 1 and 4 h post-stimulation, and the majority of the investigated immune genes (nfkb, ser and tnf) appeared up-regulated only after 24 or 48 h post- stimulation.
Octopus vulgaris, immunostimulation, hemocytes
COST Action FA1301 - CephsInAction
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Podaci o prilogu
208-208.
2015.
objavljeno
Podaci o matičnoj publikaciji
Abstract Book of the 17th International Conference on Diseases of Fish and Shellfish
Mladineo, Ivona
Las Palmas:
Podaci o skupu
17th EAFP International Conference on Diseases of Fish and Shellfish
predavanje
07.09.2015-11.09.2015
Las Palmas de Gran Canaria, Španjolska