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Pregled bibliografske jedinice broj: 880460

Effects of cell penetrating Notch inhibitory peptide conjugated to elastin-like polypeptide on glioblastoma cells


Opačak-Bernardi, Teuta; Ryu, Jung-Su; Raucher, Drazen
Effects of cell penetrating Notch inhibitory peptide conjugated to elastin-like polypeptide on glioblastoma cells // Journal of drug targeting, 25 (2017), 6; 523-531 doi:10.1080/1061186X.2017.1289537 (međunarodna recenzija, članak, znanstveni)


Naslov
Effects of cell penetrating Notch inhibitory peptide conjugated to elastin-like polypeptide on glioblastoma cells

Autori
Opačak-Bernardi, Teuta ; Ryu, Jung-Su ; Raucher, Drazen

Izvornik
Journal of drug targeting (1061-186X) 25 (2017), 6; 523-531

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cell penetrating peptide ; Notch pathway ; dnMAML ; anticancer ; elastin-like polypeptide

Sažetak
Notch pathway was found to be activated in most glioblastomas (GBMs), underlining the importance of Notch in formation and recurrence of GBM. In this study, a Notch inhibitory peptide, dominant negative MAML (dnMAML), was conjugated to elastin-like polypeptide (ELP) for tumor targeted delivery. ELP is a thermally responsive polypeptide that can be actively and passively targeted to the tumor site by localized application of hyperthermia. This complex was further modified with the addition of a cell penetrating peptide, SynB1, for improved cellular uptake and blood–brain barrier penetration. The SynB1–ELP1–dnMAML was examined for its cellular uptake, cytotoxicity, apoptosis, cell cycle inhibition and the inhibition of target genes’ expression. SynB1–ELP1–dnMAML inhibited the growth of D54 and U251 cells by inducing apoptosis and cell cycle arrest, especially in the presence of hyperthermia. Hyperthermia increased overall uptake of the polypeptide by the cells and enhanced the resulting pharmacological effects of dnMAML, showing the inhibition of targets of Notch pathway such as Hes-1 and Hey-L. These results confirm that dnMAML is an effective Notch inhibitor and combination with ELP may allow thermal targeting of the SynB1–ELP1–dnMAML complex in cancer cells while avoiding the dangers of systemic Notch inhibition.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Medicinski fakultet, Osijek

Citiraj ovu publikaciju

Opačak-Bernardi, Teuta; Ryu, Jung-Su; Raucher, Drazen
Effects of cell penetrating Notch inhibitory peptide conjugated to elastin-like polypeptide on glioblastoma cells // Journal of drug targeting, 25 (2017), 6; 523-531 doi:10.1080/1061186X.2017.1289537 (međunarodna recenzija, članak, znanstveni)
Opačak-Bernardi, T., Ryu, J. & Raucher, D. (2017) Effects of cell penetrating Notch inhibitory peptide conjugated to elastin-like polypeptide on glioblastoma cells. Journal of drug targeting, 25 (6), 523-531 doi:10.1080/1061186X.2017.1289537.
@article{article, year = {2017}, pages = {523-531}, DOI = {10.1080/1061186X.2017.1289537}, keywords = {Cell penetrating peptide, Notch pathway, dnMAML, anticancer, elastin-like polypeptide}, journal = {Journal of drug targeting}, doi = {10.1080/1061186X.2017.1289537}, volume = {25}, number = {6}, issn = {1061-186X}, title = {Effects of cell penetrating Notch inhibitory peptide conjugated to elastin-like polypeptide on glioblastoma cells}, keyword = {Cell penetrating peptide, Notch pathway, dnMAML, anticancer, elastin-like polypeptide} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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