engleski

Application of the single cell gel electrophoresis (SCGE) assay in asseeement of DNA damage in peripheral blood leukocytes of radar-facility workers

The alkaline single cell gel electrophoresis (SCGE) assay was employed to assess the Ievels of DNA damage in peripheral blood Ieukocytes of radar-facility workers daily exposed to microwave radiation and unexposed control subjects. To quantify the DNA damage the comet tail Iength and the tail moment were evaluated. In peripheral blood Ieukocytes of the exposed subjects the alkaline SCGE assay showed Iarger amount of DNA migrated, expressed by taillength and tail moment. Between the Ievels of DNA damage recorded in exposed subjects interindividual variations were also observed. Our results suggest that Iong time occupational exposure to microwave radiation could be able of causing genome damages in somatic cells and therefore it may represent a potential hazard to human health. The results obtained confirm the usefulness of the alkaline SCGE assay as a sensitive biomarker of exposure. Together with difterent cytogenetic techniques it provides a powerful technique for rapid detection of primary DNA Iesions in peripheral blood Ieukocytes of population occupationally exposed to microwave radiation.

DNA damage; radar-facility workers; microwave radiation; Ieukocytes

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