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Pregled bibliografske jedinice broj: 879746

Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli


Durajlija, Sonja; Pigac, Jasenka; Gamulin, Vera
Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli // FEMS microbiology letters, 83 (1991), 3; 317-321 doi:10.1111/j.1574-6968.1991.tb04483.x (međunarodna recenzija, članak, znanstveni)


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Naslov
Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli

Autori
Durajlija, Sonja ; Pigac, Jasenka ; Gamulin, Vera

Izvornik
FEMS microbiology letters (0378-1097) 83 (1991), 3; 317-321

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Shuttle vectors ; Plasmid stability ; Multiple cloning sites ; Streptomyces rimosus ; Streptomyces lividans ; Transfer RNA genes

Sažetak
Two bifunctional plasmid vectors pZG5 (7.45 kb) and pZG6 (6.95 kb), for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of the multicopy broad-host-range Streptomyces plasmid pIJ350 with E. coli plasmids Bluescribe M13− (pZG5) or pUC18 (pZG6). Both plasmids possess several unique restriction sites suitable for DNA cloning. Stable transformants of Streptomyces rimosus R6 and S. lividans 66 were obtained, harboring intact plasmids regardless of colony age or multiple subculturing. Moreover, pZG5 and pZG6 were successfully used to introduce several homologous transfer RNA genes into S. rimosus.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Ustanove:
Pliva-Istraživački institut,
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Jasenka Pigac (autor)

Avatar Url Vera Gamulin (autor)

Avatar Url Sonja Durajlija-Žinić (autor)

Citiraj ovu publikaciju

Durajlija, Sonja; Pigac, Jasenka; Gamulin, Vera
Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli // FEMS microbiology letters, 83 (1991), 3; 317-321 doi:10.1111/j.1574-6968.1991.tb04483.x (međunarodna recenzija, članak, znanstveni)
Durajlija, S., Pigac, J. & Gamulin, V. (1991) Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli. FEMS microbiology letters, 83 (3), 317-321 doi:10.1111/j.1574-6968.1991.tb04483.x.
@article{article, year = {1991}, pages = {317-321}, DOI = {10.1111/j.1574-6968.1991.tb04483.x}, keywords = {Shuttle vectors, Plasmid stability, Multiple cloning sites, Streptomyces rimosus, Streptomyces lividans, Transfer RNA genes}, journal = {FEMS microbiology letters}, doi = {10.1111/j.1574-6968.1991.tb04483.x}, volume = {83}, number = {3}, issn = {0378-1097}, title = {Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli}, keyword = {Shuttle vectors, Plasmid stability, Multiple cloning sites, Streptomyces rimosus, Streptomyces lividans, Transfer RNA genes} }
@article{article, year = {1991}, pages = {317-321}, DOI = {10.1111/j.1574-6968.1991.tb04483.x}, keywords = {Shuttle vectors, Plasmid stability, Multiple cloning sites, Streptomyces rimosus, Streptomyces lividans, Transfer RNA genes}, journal = {FEMS microbiology letters}, doi = {10.1111/j.1574-6968.1991.tb04483.x}, volume = {83}, number = {3}, issn = {0378-1097}, title = {Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli}, keyword = {Shuttle vectors, Plasmid stability, Multiple cloning sites, Streptomyces rimosus, Streptomyces lividans, Transfer RNA genes} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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