engleski

HLA-DRB3 polymorphism in DRB1*03 haplotypes

The DRB3 gene which encodes HLA-DR52 molecule is present on HLA-DRB1*03, *11, *12, *13 and *14 haplotypes. HLA-DRB1*03 allele is in strong LD with B*08 while A*01~B*08~DRB1*03 is the most frequent haplotype in the vast majority of the European populations. In this study we present the results of HLA-DRB3 polymorphism in unrelated individuals selected on the basis of HLA-B and DRB1 typing. All 104 studied subjects were positive for DRB1*03 and the second allele at DRB1 locus were from nonDRB3 haplotype so the DRB1*03~DRB3 haplotype could be assigned unequivocally. According to the HLA-B typing, the subjects were defined as HLA-B*08 positive or B*08 negative. HLA-A, -B, -DRB1 and -DRB3 typing was performed by PCR-SSOP (Luminex) at low or by PCR- SSP at high resolution level. In the entire group, only two out of 130 known DRB3 alleles were observed: DRB3*01:01:02 (63.5%) and DRB3*02:02:01:01 (36.5%). In the group of B*08:01 positive subjects DRB3*01:01:02 allele predominated with a frequency of 92.0% (46/50), which was highly significant in comparison with the frequency of DRB3*02:02:01:01 allele (8% ; P<0.0001). Contrary, in the group of HLA-B*08:01 negative subjects DRB3*02:02:01:01 allele was found more frequently (63.0%) than DRB3*01:01:02 (37.0%) and the difference was also statistically significant (P=0.012). Further analysis in this group revealed that B*44:02:01G is more frequently found in DRB1*03~DRB3*01:01:02 haplotype. Analysis of the association with HLA-A alleles in any of the studied groups, did not show statistically significant difference. These results will be useful in further population and disease association studies as well as implemented in DSA monitoring.

HLA-DRB3, haplotypes

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