engleski

Electrochemical and spectrophotometric determination of total polyphenol content in Croatian apple varieties

Oxidation mechanism of quercetin in three different electrolytes (KCl, NaCl and LiCl) was studied by cyclic and differential pulse voltammetry. The results have shown that quercetin oxidation is reversible and diffusion controlled process in all three investigated electrolytes. Calibration diagram of quercetin was obtained in KCl, so this electrolyte was selected for total polyphenol content determination in apple peel extracts. Total polyphenol content was analysed by differential pulse voltammetry (dpv) and spectrophotometric Folin-Ciocalteu method (FC) in several ancient Croatian apple varieties and a wild apple variety grown in Croatia by using quercetin as a standard. Very high correlation for TP content obtained by two methods was observed since the TPdpv/TPFC ratio was around 1 and a correlation coefficient (R2) for TPdpv vs. TPFC was 0.98. The highest total polyphenol content was found in Slavonska srčika variety.

apple extracts ; differential pulse voltammetry ; spectrophotometry ; quercetin ; polyphenol determination

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