engleski

Effects of between generations changes in nutrition type on adipokine levels in female Sprague Dawley rat

Introduction: Previous studies established that between generations changes in feeding protocol can have significant impact on physiology of adipose tissue. The aim of the study was to determinate effects of mothers` nutrition and nutrition of the offspring on circulation adipokine levels in all groups of rats. Methods: Ten female rats were randomly divided in two groups, first group fed with food containing high content of saturated fatty acids (hfd) and the second with standard laboratory chow (cd). After coupling and lactation period their offspring were further randomly divided into two subgroups fed hfd or cd forming four study groups: a) cd-cd, b) cd- hfd, c) hfd-cd and d) hfd-hfd. The dams and offspring at the age of 37 and 18 weeks, respectively, were subjected to biochemical analysis of the blood adiponectin, il-6 and tnf-α levels. Additionally body weight was recorded and bmi calculated. Results: There was no significantdifference in body weight and bmi between groups. Higher il-6 concentration was measured in hfd group of dams (P = 0.009), as well as lower adiponectin concentration (P = 0.009). Significantly higher concentrations of il-6 and tnf-α were observed in hfdhfd and hfd- cd groups (P = 0.004 and P = 0.004, respectively), as well as low adiponectin level (P = 0.004). Conclusion: High fat diet consuption is not necessary correlated with high body weight and bmi. Nevertheless, maternal hfd consumption predisposes offspring to increased risk of disturbances in adipokine levels resulting in metabolic abnormalities

obesity, rat, adipokine, offspring

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