engleski

Phytochemical composition and biological activity of the extracts of Satureja subspicata Vis. growing in Bosnia and Herzegovina

Satureja subspicata Vis. (mountain savory) is a rare, endemic species distributed in the eastern Mediterranean area [1]. In traditional medicine of Bosnia and Herzegovina (BiH) S. subspicata is used to treat leukemia and syndromes of lymphatic nodes since long ago. In this area preparations of S. montana and S. subspicata have been used with excellent results for different cardiovascular disorders, especially arrhythmia, heart fibrillation and the diseases of blood vessels [1]. There are only a few studies about the chemical composition and biological activity of non-volatile extracts of S. subspicata growing in Croatia and none report on the extracts fom BiH. In this work we investigated the phytochemical composition of methanolic and hot water extracts of S. subspicata collected in BiH, total phenols and flavonoids. In addition, the aim of this work was to determine the antioxidant potential and inhibition of cholinesterases. Chemical composition of extracts was assessed using HPLC/DAD technique. Antioxidant capacity was determined using DPPH, FRAP methods [3-4] and by determining activity of extracts in linoleic acid emulsion [5]. Using Ellman method inhibition of acetilcholinesterase and butyrylcholinesterase was determined [6]. The major compounds found in methanolic extract where: rosmarinic acid, eriodictyol and kaempferol, while in hot water extract major compounds where: rosmarinic acid, myricetin and ferulic acid. Obtained results for content of total phenols and total flavonoids indicate considerable amount of these compounds. In concentration of 1 g/L in stock solution extracts showed low inhibition of both cholinesterases and good antioxidant potential.

Satureja subspicata ; phytochemical composition ; biological activity ; HPLC

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