Qualitative HPLC-DAD-ESI-TOF-MS analysis, cytotoxic and apoptotic effects of Croatian endemic Centaurea ragusina L. aqueous extracts (CROSBI ID 239780)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Radan, Mila ; Carev, Ivana ; Tešević, Vele ; Politeo, Olivera ; Čikeš Čulić, Vedrana
engleski
Qualitative HPLC-DAD-ESI-TOF-MS analysis, cytotoxic and apoptotic effects of Croatian endemic Centaurea ragusina L. aqueous extracts
Centaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical constituents were tentatively identified using high performance liquid chromatography HPLC-DAD-ESI-TOF-MS in negative ionization mode. The main compounds of herba extract were sesquiterpene lactones: solstitialin A-3, 13 diacetate and epoxyrepdiolide ; organic acid: quinic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid and malic acid ; sesquiterpene lactone: cynaropicrin ; phenolic compounds: chlorogenic acid and phenylpropanoid: syringin. The aqueous extracts of C. ragusina were investigated for correlation of their effects on human bladder (T24) and human glioblastoma (A1235) cancer cell lines using the MTT assay. Although both extracts showed significant dose- and time-dependent cytotoxicactivity against both cancer cell lines, the flower extract exhibited slightly higher activity. In order to determine type of cell death induced by treatment, cell lines were exposed subsequently to a treatment with both flower and herba aqueous extracts. The majority of the cells died by induced apoptosis treatment. Flower aqueous extract (26.25 %), compared to a leaf aqueous extract (22.15 %) showed slightly higher percentage of an apoptosis in T24 cells, when compared to a non-treated cells (0.04 %).
Centaurea ragusina ; cytotoxic ; apoptotic ; MTT ; sesquiterpene lactone
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
14 (9)
2017.
e1700099
9
objavljeno
1612-1872
1612-1880
10.1002/cbdv.201700099