engleski

ICP-OES analysis of silver in tobacco seedlings (Nicotiana tabacum) treated with silver

In the last few years a remarkable progress has been made in the domain of nanotechnology. This discovery led to many commercial applications that involve the use of variety of nanoparticles. [1] Most widely commercially used are silver nanoparticles (AgNPs). Although there are benefits that come with their applicability, there is an increasing concern about their impact on the environment, especially plants. In order to better understand the interactions between AgNPs and plants and to differentiate between ionic and particle specific silver, morphological and proteomic changes were studied.[2] In this work we used inductively coupled plasma-optical emission spectrometry (ICP-OES) for quantitative determination of silver content in Nicotiana tabacum seedlings which were exposed to AgNPs stress. Working parameters of instrument were adjusted using the most sensitive silver emission line of 328.068 nm. Detection limit (1.5 ng g-1) at higher RF power of 1.3 kW was determined using method blank which consisted of growth medium matrix. The analytical method required conversion of a dried plant sample into aqueous solution. Digestion of plant samples was performed using acid digestion (HNO3, Spectrapure 70 % v/v ; H2O2, ultrapure 30 % v/v) assisted by microwave irradiation. Concentrations of Ag in plant seedlings, growth medium and residual precipitate were successfully determined in the concentration range of Ag 2.5 -18 µg g-1. Precision (RSD < 1.0 %) and accuracy were determined using certified reference material (NIST-1573a CRM Tomato Leaves).

ICP-AES, Silver

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