engleski

Influence of temperature and moisture on toxicity of propiconazole and chlorantraniliprole to earthworm Eisenia fetida

Earthworms became the most used biological model in toxicity assays and environmental monitoring studies, particularly for the toxicity of pesticides on soil ecosystems. Although some pesticides are reported safe for non-target invertebrates there are not tested with possibility of soil temperature and moisture regime change. Results of many studies show a general warming in all seasons and soil depths in the observed periods for soil temperatures, with change in soil moisture as well. The aim of this study is to investigate the biochemical responses of earthworm Eisenia fetida and its gut microbial activity after exposure to propiconazole (PCZ) and chlorantraniliprole (CAP) under different temperature and moisture regimes. Chosen temperatures were 20 C and 25 C and soil moisture 30% and 50%. Activities of acetylcholinesterase (AChE), catalase (CAT) and glutathione-S-transferase (GST) were measured after 7 days of exposure and, after transfer to clean soil, 1, 3, 5 and 10 days post exposure. Additionally, gut microbial activity was investigated by conducting crystal violet (CV) test and dehydrogenase activity (INT) measuring on earthworm faeces obtain after exposure and each post-exposure sampling. Some of the results were as expected considering the stress elicited by higher temperature and low soil moisture, but the increased activity of acetylcholinesterase obtained after exposure was not expected.

climate change, AChE, CAT, GST

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