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Pregled bibliografske jedinice broj: 87572

Presence and activation of nuclear phosphoinositide 3-kinase C2beta during compensatory liver growth.


Sinđić, Aleksandra; Aleksandrova, Aleksandra; Alan P., Fields; Stefano, Volinia; Banfić, Hrvoje
Presence and activation of nuclear phosphoinositide 3-kinase C2beta during compensatory liver growth. // The Journal of Biological Chemistry, 276 (2001), 21; 17754-17761 (međunarodna recenzija, članak, znanstveni)


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Naslov
Presence and activation of nuclear phosphoinositide 3-kinase C2beta during compensatory liver growth.

Autori
Sinđić, Aleksandra ; Aleksandrova, Aleksandra ; Alan P., Fields ; Stefano, Volinia ; Banfić, Hrvoje

Izvornik
The Journal of Biological Chemistry (1083-351X) 276 (2001), 21; 17754-17761

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
compensatory liver growth; liver nuclei; phosphoinositide 3-kinase C2beta; phosphatidylinositol 4-phosphate; phosphatidylinositol 4;5-bisphosphate; phosphatidylinositol 3;4;5-trisphosphate

Sažetak
Highly purified liver nuclei incorporated radiolabeled phosphate into phosphatidylinositol 4-phosphate (PtdIns(4)P), PtdIns(4,5)P(2), and PtdIns(3,4,5)P(3). When nuclei were depleted of their membrane, no radiolabeling of PtdIns(3,4,5)P(3) could be detected showing that within the intranuclear region there are no class I phosphoinositide 3-kinases (PI3K)s. In membrane-depleted nuclei harvested 20 h after partial hepatectomy, the incorporation of radiolabel into PtdIns(3)P was observed together with an increase in immunoprecipitable PI3K-C2beta activity, which is sensitive to wortmannin (10 nm) and shows strong preference for PtdIns over PtdIns(4)P as a substrate. On Western blots PI3K-C2beta revealed a single immunoreactive band of 180 kDa, whereas 20 h after partial hepatectomy gel shift of 18 kDa was noticed, suggesting that observed activation of enzyme is achieved by proteolysis. When intact membrane-depleted nuclei were subjected to short term (20 min) exposure to micro-calpain, similar gel shift together with an increase in PI3K-C2beta activity was observed, when compared with the nuclei harvested 20 h after partial hepatectomy. Moreover, the above-mentioned gel shift and increase in PI3K-C2beta activity could be prevented by the calpain inhibitor calpeptin. The data presented in this report show that, in the membrane-deplet nuclei during the compensatory liver growth, there is an increase in PtdIns(3)P formation as a result of PI3K-C2beta activation, which may be a calpain-mediated event.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
108013

Ustanove:
Medicinski fakultet, Zagreb


Citiraj ovu publikaciju:

Sinđić, Aleksandra; Aleksandrova, Aleksandra; Alan P., Fields; Stefano, Volinia; Banfić, Hrvoje
Presence and activation of nuclear phosphoinositide 3-kinase C2beta during compensatory liver growth. // The Journal of Biological Chemistry, 276 (2001), 21; 17754-17761 (međunarodna recenzija, članak, znanstveni)
Sinđić, A., Aleksandrova, A., Alan P., F., Stefano, V. & Banfić, H. (2001) Presence and activation of nuclear phosphoinositide 3-kinase C2beta during compensatory liver growth.. The Journal of Biological Chemistry, 276 (21), 17754-17761.
@article{article, author = {Sin\dji\'{c}, Aleksandra and Aleksandrova, Aleksandra and Alan P., Fields and Stefano, Volinia and Banfi\'{c}, Hrvoje}, year = {2001}, pages = {17754-17761}, keywords = {compensatory liver growth, liver nuclei, phosphoinositide 3-kinase C2beta, phosphatidylinositol 4-phosphate, phosphatidylinositol 4, 5-bisphosphate, phosphatidylinositol 3, 4, 5-trisphosphate}, journal = {The Journal of Biological Chemistry}, volume = {276}, number = {21}, issn = {1083-351X}, title = {Presence and activation of nuclear phosphoinositide 3-kinase C2beta during compensatory liver growth.}, keyword = {compensatory liver growth, liver nuclei, phosphoinositide 3-kinase C2beta, phosphatidylinositol 4-phosphate, phosphatidylinositol 4, 5-bisphosphate, phosphatidylinositol 3, 4, 5-trisphosphate} }
@article{article, author = {Sin\dji\'{c}, Aleksandra and Aleksandrova, Aleksandra and Alan P., Fields and Stefano, Volinia and Banfi\'{c}, Hrvoje}, year = {2001}, pages = {17754-17761}, keywords = {compensatory liver growth, liver nuclei, phosphoinositide 3-kinase C2beta, phosphatidylinositol 4-phosphate, phosphatidylinositol 4, 5-bisphosphate, phosphatidylinositol 3, 4, 5-trisphosphate}, journal = {The Journal of Biological Chemistry}, volume = {276}, number = {21}, issn = {1083-351X}, title = {Presence and activation of nuclear phosphoinositide 3-kinase C2beta during compensatory liver growth.}, keyword = {compensatory liver growth, liver nuclei, phosphoinositide 3-kinase C2beta, phosphatidylinositol 4-phosphate, phosphatidylinositol 4, 5-bisphosphate, phosphatidylinositol 3, 4, 5-trisphosphate} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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