engleski

Release of Trichoderma viride spores from microcapsules simultaneously loaded with chemical and biological agents

Recent studies of simultaneous encapsulation of chemical and biological active agents in chitosan/alginate microcapsules revealed that encapsulation of Trichoderma viride spores and copper cations in the same compartment do not inhibit their activity. It has been demonstrated a synergistic effect of simultaneously encapsulated biological and chemical active agents in produced biopolymer microcapsules.The objective of this work was to investigate an influence of formulation variables (concentration of copper cation, chitosan layer and microcapsule size) on Trichoderma viride releasing. Releasing of Trichoderma viride strongly depend on copper cation concentration, microcapsule size and the presence of chitosan layer on microcapsule surface. The increase in copper cation concentration promoted, but the increase in microcapsule size and presence of chitosan layer on microcapsule surface reduced Trichoderma viride releasing. A set of release profiles exhibited rapid initial burst followed by slower release except those large microcapsules with chitosan layer, which show the initial lag time. Fitting to simple Korsmeyer-Peppas empirical model elucidated the underlying mechanism of Trichoderma viride releasing. Fickian diffusion controlled release from microcapsules without chitosan layer and smaller microcapsules with chitosan layer, whereas anomalous diffusion mechanism (a combination of the diffusion and erosion mechanisms) was found to be the rate-controlling mechanism of release from larger microcapsules with chitosan layer. The investigation revealed that proper selection of formulation variables help us in designing microcapsules with controlled release of bioactive agents for plant protection and nutrition.

sodium alginate, chitosan, copper, Trichoderm viride, controlled release

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