engleski

Construction of novel Saccharomyces cerevisiae hybrid diploids for bioethanol production

Bioethanol production from raw lignocellulosic material requires a producer strain which tolerates high ethanol concentrations and presence of growth and fermentation inhibitors. Therefore we constructed hybrid diploid S. cerevisiae strains by mating two haploids having desirable traits. One haploid was isolated from wine thus tolerating high ethanol concentration while the other has been shown to be resistant to several growth and fermentation inhibitors. These haploids were mated and resulting homozygous (control) and heterozygous (hybrid) diploids were tested for their ability to grow in the presence of several inhibitors frequently found in different lignocellulosic hydrolysates (acetic and levulinic acid, 2- furaldehyde) and to produce ethanol. The results showed that some hybrid diploids were more resistant to inhibitors than parental haploid strains or homozygous diploids. Interestingly, constructed hybrids showed different fermentation ability in CO2 production test, suggesting genetic variability of constructed strains. All together, these results suggest that the approach used in this study may help to construct new strains as well as to improve traits of biotechnologically interesting strains.

hybrid diploids, bioethanol production

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