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THE EFFECT OF NATURAL ANTIOXIDANTS IN THIYL RADICAL-INDUCED LIPID MODIFICATION PROCESSES


Tartaro Bujak, Ivana; Mihaljević, Branka
THE EFFECT OF NATURAL ANTIOXIDANTS IN THIYL RADICAL-INDUCED LIPID MODIFICATION PROCESSES // ICARST 2017-International Conference on Applications of radiation Science and Technology
Vienna: International Atomic Energy Agency, 2017. str. 413-413 (poster, nije recenziran, sažetak, znanstveni)


Naslov
THE EFFECT OF NATURAL ANTIOXIDANTS IN THIYL RADICAL-INDUCED LIPID MODIFICATION PROCESSES

Autori
Tartaro Bujak, Ivana ; Mihaljević, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ICARST 2017-International Conference on Applications of radiation Science and Technology / - Vienna : International Atomic Energy Agency, 2017, 413-413

Skup
International Conference on Applications of radiation Science and Technology

Mjesto i datum
Beč, Austrija, 24-28.04.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Fatty acids, micelles, lipid peroxidation, geometrical isomerization

Sažetak
Background of the study. Polyunsaturated fatty acids (PUFA), major constituents of biological membranes are sensitive to a free radical attack. The reactions of PUFA with free radicals are known to occur via two main processes: (i) lipid peroxidation and (ii) cis– trans isomerization. Lipid peroxidation can be inhibited by thiols, due to their H-donation ability. On the other hand, thiyl radicals are known to catalyse the double bond isomerization in PUFA. Both processes have damaging potential that must be carefully considered for its consequences in a biological systems. Therefore, the protection against lipid degradation under oxidative and free radical conditions is of special interest. The aim of this study is to elucidate the influence of different naturally occurring antioxidants on lipid peroxidation and cis–trans isomerization processes in biomimetic model system under different conditions. Methodology. Model system containing mixed surfactant micelles and buffer was prepared by slow solubilization of linoleic acid (LH) in non-ionic surfactant micelles previously formed by mixing Tween®-20 and PB, pH 6.5. The composition of the investigated systems was typically 0.5 mM LH, 0.28 mM Tween®-20 and 5 mM PB (pH ~ 5). Ascorbic acid (AscH), -tocopherol (α-TOH) and resveratrol (ResOH)) of defined concentrations were added during preparation of model systems. The addition of the amphiphilic 2.8 mM 2- mercaptoethanol was added to previously prepared micelles just before irradiation. Model lipid systems were irradiated up to the dose of 400 Gy, in equilibrium with air or after saturation with N2O at room temperature using panoramic 60Co source at the Ruđer Bošković Institute. Accurate dose rates in the irradiating positions were established with the ethanol-chlorobenzene dosimetry system (ISO/ASTM 51538:2009). The concentrations of hydroperoxides of linoleic acid were determined by the spectrophotometric ferric thiocyanate method, and the geometrical isomerization of LH methyl ester was studied by capillary gas chromatography. Results. Under air-equlibrated conditions, the addition of different natural occuring antioxidants retarded the process of lipid peroxidation among which ResOH showed the best antioxidative property. In model systems where process of cis–trans isomerization prevailed, the presence of antioxidants influenced on a decrease of the trans isomerization level ; AscH was the the most effective inhibitor of radiation-induced trans- isomer formation. Among the natural compounds analyzed in this work an amphiphilic ResOH has proven to be the most effective antioxidant, also significant inhibitor for cis–trans isomerization process in conditions of low irradiation dose rates with formation of low initial radical species concentration. Conclusion. Results indicate that the cis–trans isomerization and lipid peroxidation processes level could be dependent on the hydrophilic/lipophilic properties of particular antioxidant and its localization in model lipid system. This study contributes to the understanding of the role of antioxidants in radical processes which are not only able to prevent peroxidation but can also behave as anti-isomerizing compounds.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb