Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi !

The effect of adjuvants on immune reaction to snake venoms (CROSBI ID 484590)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Lang Balija, Maja ; Halassy Špoljar, Beata ; Dojnović, Biserka ; Vrdoljak, Anto ; Tomašić, Jelka ; Vranešić, Branka The effect of adjuvants on immune reaction to snake venoms // Third World Congress on Vaccines and Immunisation / Kurstak Edouard (ur.). Infections Control World Organisation, 2002. str. 84-84-x

Podaci o odgovornosti

Lang Balija, Maja ; Halassy Špoljar, Beata ; Dojnović, Biserka ; Vrdoljak, Anto ; Tomašić, Jelka ; Vranešić, Branka

engleski

The effect of adjuvants on immune reaction to snake venoms

Long-nosed viper (V. ammodytes) is one of the most poisonous snakes, that is widely distributed through central Europe. Specific antiserum, that is produced by gradual immunisation of big animals (horse, donkey, sheep), is the only available therapeutic drug for curing snake venom poisoning. The aim of our work was to establish a mice model for studying immune reaction (IR) specific for the snake venom, that is a toxin and an immunogen at the same time, and to monitor the influence of experimental adjuvants, peptidoglycan monomer GlcNAc-MurNAc-L-Ala-D-isoGln-mesoDAP(wNH2)-D-Ala-D-Ala (PGM) and L-(adamant-2-il)-Gly-L-Ala-D-isoGln (AdTP2), on this reaction. Optimal immunisation protocol was found by varying the amount of the venom dose and the schedule of immunisation. The amount of the antigen was scaled down starting with the maximal quantity of the venom that is not subcutaneously lethal for mice. The immunisation schedule, we found to be optimal, included four s.c. immunisations in three week intervals. The specific IR was monitored by ELISA measuring venom-specific IgGs (qualitatively and quantitatively). The selected sera with high titres of specific antibodies were tested for their ability to neutralise snake venom. The stimulatory effect of adjuvants, PGM and AdTP2, was demonstrated using suboptimal doses of venom.

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

84-84-x.

2002.

objavljeno

Podaci o matičnoj publikaciji

Third World Congress on Vaccines and Immunisation

Kurstak Edouard

Infections Control World Organisation

Podaci o skupu

Third World Congress on Vaccines and Immunisation

poster

04.06.2002-09.06.2002

Opatija, Hrvatska

Povezanost rada

Kliničke medicinske znanosti