engleski

Antisense RNA silencing of the TROL protein involved in plant photosynthetic energy partitioning

Although basic mechanisms of plant photosynthesis have been established, regulatory pathways of energy partitioning are still largely unknown. We have proposed that At4g01050 gene product (TROL protein) from Arabidopsis thaliana (L.) Heynh., due to its catalytically inactive rhodanese domain and FNR binding, participates in regulation of photosynthetic electron partitioning (1). Many know catalytically inactive rhodanese domains are implicated in regulation, most commonly linked with signalling processes. Here we present reverse genetic approach to study the functions of the At4g01050 gene by using antisense RNA silencing. The At4g01050 cDNA was inserted into pGPTVBAR binary vector in the antisense orientation, with respect to CaMV 35S promoter. Arabidopsis plants were subsequently transformed with pGPTV-antiTROL construct by using agroinfiltration. Plants with silenced TROL were subsequently analysed on molecular, biochemical, and cell biology levels (2). References: 1. L. Vojta, L. D. Carić, V. Cesar, J. Antunović Dunić, H. Lepeduš, M. Kveder, H. Fulgosi: TROL-FNR interaction reveals alternative pathways of electron partitioning in photosynthesis. Scientific Reports 5, 10085 ; doi: 10.1038/srep10085 (2015). 2. S. Jurić, K. Hazler Pilepić, A. Tomašić, H. Lepeduš, B. Jeličić, S. Puthiyaveetil, T. Bionda, L. Vojta, J. F. Allen, E. Schleiff, H. Fulgosi: Tethering of ferredoxin:NADP+ oxidoreductase to thylakoid membranes is mediated by novel chloroplast protein TROL. The Plant Journal. 60 (2009): 783-794.

ferredoxin NADAP+ oksidoreductase ; photosystem I ; agrobacterium-mediated plant transformation ; Arabidopsis thaliana

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