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Pregled bibliografske jedinice broj: 872822

What a difference a methyl group makes - the selectivity of monoamine oxidase B towards histamine and N-methylhistamine


Maršavelski, Aleksandra; Vianello, Robert
What a difference a methyl group makes - the selectivity of monoamine oxidase B towards histamine and N-methylhistamine // Chemistry : a European journal, 23 (2017), 12; 2915-2925 doi:10.1002/chem.201605430 (međunarodna recenzija, članak, znanstveni)


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Naslov
What a difference a methyl group makes - the selectivity of monoamine oxidase B towards histamine and N-methylhistamine

Autori
Maršavelski, Aleksandra ; Vianello, Robert

Izvornik
Chemistry : a European journal (0947-6539) 23 (2017), 12; 2915-2925

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
MAO B, MM-PBSA, MD, QM, histamine, N-methylhistamine

Sažetak
Monoamine oxidase (MAO) enzymes catalyze the degradation of a very broad range of biogenic and dietary amines including many neurotransmitters in the brain, whose imbalance is extensively linked with the biochemical pathology of various neurological disorders. Although sharing around 70% sequence identity, both MAO A and B isoforms differ in substrate affinities and inhibitor sensitivities. Inhibitors that act on MAO A are used to treat depression, due to their ability to raise serotonin concentrations, while MAO B inhibitors decrease dopamine degradation and improve motor control in patients with Parkinson disease. Despite this functional importance, the contributions affecting MAO selectivity are poorly understood. Here we used a combination of MD simulations, MM–PBSA binding free energy evaluations, and QM cluster calculations to address the unexpected, yet challenging MAO B selectivity for N-methylhistamine (NMH) over histamine (HIS), differing only in a single methyl group distant from the reactive ethylamino centre. We show that a dominant selectivity contribution is offered by a lower activation free energy for NMH by 2.6 kcal mol–1, in excellent agreement with the experimental ΔΔG‡ EXP = 1.4 kcal mol–1, together with a more favourable reaction exergonicity and active site binding. This study also confirms the hydrophobic nature of the MAO B active site and underlines the important role of Ile199, Leu171 and Leu328 in properly orienting substrates for the reaction.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Ustanove:
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Maršavelski, Aleksandra; Vianello, Robert
What a difference a methyl group makes - the selectivity of monoamine oxidase B towards histamine and N-methylhistamine // Chemistry : a European journal, 23 (2017), 12; 2915-2925 doi:10.1002/chem.201605430 (međunarodna recenzija, članak, znanstveni)
Maršavelski, A. & Vianello, R. (2017) What a difference a methyl group makes - the selectivity of monoamine oxidase B towards histamine and N-methylhistamine. Chemistry : a European journal, 23 (12), 2915-2925 doi:10.1002/chem.201605430.
@article{article, year = {2017}, pages = {2915-2925}, DOI = {10.1002/chem.201605430}, keywords = {MAO B, MM-PBSA, MD, QM, histamine, N-methylhistamine}, journal = {Chemistry : a European journal}, doi = {10.1002/chem.201605430}, volume = {23}, number = {12}, issn = {0947-6539}, title = {What a difference a methyl group makes - the selectivity of monoamine oxidase B towards histamine and N-methylhistamine}, keyword = {MAO B, MM-PBSA, MD, QM, histamine, N-methylhistamine} }
@article{article, year = {2017}, pages = {2915-2925}, DOI = {10.1002/chem.201605430}, keywords = {MAO B, MM-PBSA, MD, QM, histamine, N-methylhistamine}, journal = {Chemistry : a European journal}, doi = {10.1002/chem.201605430}, volume = {23}, number = {12}, issn = {0947-6539}, title = {What a difference a methyl group makes - the selectivity of monoamine oxidase B towards histamine and N-methylhistamine}, keyword = {MAO B, MM-PBSA, MD, QM, histamine, N-methylhistamine} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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