engleski

COMPARATIVE CHEMICAL COMPOSITION, ANTI-PROLIFERATIVE AND APOPTOTIC ACTIVITY OF CROATIAN ENDEMIC CENTAUREA RAGUSINA L. AQUEOUS EXTRACTS

The Centaurea genus species herbal remedies have been used in ethnomedicines in Mediterranean area for treatment of various diseases as they possess a valuable source of bioactive compounds. Sesquiterpene lactones, phenols, flavonoids and lignans are usually regarded as a group of compounds responsible for diverse biological activity of Centaurea plant extracts The chemical constituents were obtained and tentatively identified using high performance liquid chromatography HPLC-DAD-ESI-TOF-MS in negative ionisation mode. The aqueous extracts of C. ragusina were investigated for correlation of their effects on human bladder (T24) and human glioblastomma (A1235) cancer cell lines using the MTT assay. Cells were exposed subsequently to both extracts in order to determine type of cell death induced by treatment. The main compounds of herba extract were sesquiterpene lactones: solstitialin A-3, 13 diacetate and epoxyrepdiolide ; quinic acid as organic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid, malic acid ; sesquiterpene lactone cynaropicrin ; phenolic compounds: chlorogenic acid and phenylpropanoid syringin. Although both extracts showed significant dose- and time-dependent cytotoxicity against both cancer cell lines, the flower extract exhibited slightly better activity. The majority of the cells died by treatment induced apoptosis. Flower extract (26.25%) compared to a leaf extract (22.15%) showed slightly higher percentage of an apoptosis in T24 cells compared to non-treated cells (3.27 %). Centaurea ragusina L., an endemic Croatian plant species, revealed a good anti- proliferative activity of aqueous extracts on human bladder (T24) and human glioblastomma (A1235) cancer cell lines. Centaurea ragusina aqueous extract represents a valuable source of sesquiterpene lactones and flavonoids with anticancer properties reported in numerous studies.

Centaurea ; sesquiterpene ; MTT assay

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