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Chemical composition and biological activity of volatile oil from wild edible Mediterranean plant Centaurea scabiosa

Centaurea scabiosa is wild edible plant used in food and nutrition in Mediterranean area. A whole juvenile plant is used in local cuisine of Dalmatian region, Croatia. Volatile oils may comprise variety of biologically active compounds. Aim of this work was to investigate the volatile oil chemistry of C. scabiosa species and its biological activity: antioxidant potential, inhibition of cholinesterases and toxicity on human cells. The chemical composition of essential oil was assessed using GC/MS technique. Antioxidant capacity of essential oil was determined using DPPH and FRAP methods. Inhibition of both acetylcholinesterase and butyrylcholinesterase was determined using Ellman method. Toxicity of C. scabiosa volatile oil was measured on primary fibroblasts through the MTT assay. [11] The major compounds found in essential oil were heptacosane, caryophyllene oxide, alloaromadendrene epoxide, α-cyperone and α-bisabolol. Essential oil in stock concentration of 1g/L showed moderate inhibition of both cholinesterases while antioxidant potential was low on both tested methods. C. scabiosa volatile oil showed no toxicity on primary fibroblasts at the highest tested dose of 0, 125g/L in 0, 5% DMSO.

Centaurea scabiosa, essential oil, GC/MS, biological activity

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