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Pregled bibliografske jedinice broj: 870680

Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins


Rogov, Vladimir V.; ...; Marinković, Mija; Lang, Verena; ...; Buljubašić, Maja; Šprung, Matilda; ...; Dikić, Ivan; ...; Novak, Ivana
Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins // Scientific Reports, 7 (2017), 1131-1 doi:10.1038/s41598-017-01258-6 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 870680 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins

Autori
Rogov, Vladimir V. ; ... ; Marinković, Mija ; Lang, Verena ; ... ; Buljubašić, Maja ; Šprung, Matilda ; ... ; Dikić, Ivan ; ... ; Novak, Ivana

Izvornik
Scientific Reports (2045-2322) 7 (2017); 1131-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Nix, LIR phosphorylation, LC3, mitophagy

Sažetak
The mitophagy receptor Nix interacts with LC3/GABARAP proteins, targeting mitochondria into autophagosomes for degradation. Here we present evidence for phosphorylation-driven regulation of the Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Substitution of Lys51 to Ala in LC3B abrogates binding of a phosphomimetic Nix mutant. Functionally, serine 34/35 phosphorylation enhances autophagosome recruitment to mitochondria in HeLa cells. Together, this study provides cellular, biochemical and biophysical evidence that phosphorylation of the LIR domain of Nix enhances mitophagy receptor engagement.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
HRZZ-UIP-2013-11-5246 - Uloga receptora autofagije u selektivnom uklanjanju mitohondrija (AutoMito) (Novak Nakir, Ivana, HRZZ - 2013-11) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Split,
Medicinski fakultet, Split

Profili:

Avatar Url Matilda Šprung (autor)

Avatar Url Ivana Novak (autor)

Avatar Url Mija Marinković (autor)

Avatar Url Maja Buljubašić (autor)

Citiraj ovu publikaciju

Rogov, Vladimir V.; ...; Marinković, Mija; Lang, Verena; ...; Buljubašić, Maja; Šprung, Matilda; ...; Dikić, Ivan; ...; Novak, Ivana
Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins // Scientific Reports, 7 (2017), 1131-1 doi:10.1038/s41598-017-01258-6 (međunarodna recenzija, članak, znanstveni)
Rogov, V., ..., Marinković, M., Lang, V., ..., Buljubašić, M., Šprung, M., ..., Dikić, I., ... & Novak, I. (2017) Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins. Scientific Reports, 7, 1131-1 doi:10.1038/s41598-017-01258-6.
@article{article, year = {2017}, pages = {1131-1-1131-}, DOI = {10.1038/s41598-017-01258-6}, keywords = {Nix, LIR phosphorylation, LC3, mitophagy}, journal = {Scientific Reports}, doi = {10.1038/s41598-017-01258-6}, volume = {7}, issn = {2045-2322}, title = {Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins}, keyword = {Nix, LIR phosphorylation, LC3, mitophagy} }
@article{article, year = {2017}, pages = {1131-1-1131-}, DOI = {10.1038/s41598-017-01258-6}, keywords = {Nix, LIR phosphorylation, LC3, mitophagy}, journal = {Scientific Reports}, doi = {10.1038/s41598-017-01258-6}, volume = {7}, issn = {2045-2322}, title = {Phosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins}, keyword = {Nix, LIR phosphorylation, LC3, mitophagy} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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