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Pregled bibliografske jedinice broj: 870638

Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis


Kalantari, Aida; Chen, Tao; Ji, Boyang; Stancik, Ivan A.; Ravikumar, Vaishnavi; Franjevic, Damjan; Saulou-Bérion, Claire; Goelzer, Anne; Mijakovic, Ivan
Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis // Frontiers in Microbiology, 8 (2017), 638; 1-11 doi:10.3389/fmicb.2017.00638 (međunarodna recenzija, članak, znanstveni)


Naslov
Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis

Autori
Kalantari, Aida ; Chen, Tao ; Ji, Boyang ; Stancik, Ivan A. ; Ravikumar, Vaishnavi ; Franjevic, Damjan ; Saulou-Bérion, Claire ; Goelzer, Anne ; Mijakovic, Ivan

Izvornik
Frontiers in Microbiology (1664-302X) 8 (2017), 638; 1-11

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
3-hydroxypropanoic acid ; glycerol ; Bacillus subtilis ; metabolic engineering ; glycerol kinase knock-out

Sažetak
3-Hydroxypropanoic acid (3-HP) is an important biomass-derivable platform chemical that can be converted into a number of industrially relevant compounds. There have been several attempts to produce 3-HP from renewable sources in cell factories, focusing mainly on Escherichia coli, Klebsiella pneumoniae, and Saccharomyces cerevisiae. Despite the significant progress made in this field, commercially exploitable large-scale production of 3-HP in microbial strains has still not been achieved. In this study, we investigated the potential of Bacillus subtilis as a microbial platform for bioconversion of glycerol into 3-HP. Our recombinant B. subtilis strains overexpress the two-step heterologous pathway containing glycerol dehydratase and aldehyde dehydrogenase from K. pneumoniae. Genetic engineering, driven by in silico optimization, and optimization of cultivation conditions resulted in a 3-HP titer of 10 g/L, in a standard batch cultivation. Our findings provide the first report of successful introduction of the biosynthetic pathway for conversion of glycerol into 3-HP in B. subtilis. With this relatively high titer in batch, and the robustness of B. subtilis in high density fermentation conditions, we expect that our production strains may constitute a solid basis for commercial production of 3-HP.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Ustanove
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Ivan Mijaković (autor)

Avatar Url Damjan Franjević (autor)

Citiraj ovu publikaciju

Kalantari, Aida; Chen, Tao; Ji, Boyang; Stancik, Ivan A.; Ravikumar, Vaishnavi; Franjevic, Damjan; Saulou-Bérion, Claire; Goelzer, Anne; Mijakovic, Ivan
Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis // Frontiers in Microbiology, 8 (2017), 638; 1-11 doi:10.3389/fmicb.2017.00638 (međunarodna recenzija, članak, znanstveni)
Kalantari, A., Chen, T., Ji, B., Stancik, I., Ravikumar, V., Franjevic, D., Saulou-Bérion, C., Goelzer, A. & Mijakovic, I. (2017) Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis. Frontiers in Microbiology, 8 (638), 1-11 doi:10.3389/fmicb.2017.00638.
@article{article, year = {2017}, pages = {1-11}, DOI = {10.3389/fmicb.2017.00638}, keywords = {3-hydroxypropanoic acid, glycerol, Bacillus subtilis, metabolic engineering, glycerol kinase knock-out}, journal = {Frontiers in Microbiology}, doi = {10.3389/fmicb.2017.00638}, volume = {8}, number = {638}, issn = {1664-302X}, title = {Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered Bacillus subtilis}, keyword = {3-hydroxypropanoic acid, glycerol, Bacillus subtilis, metabolic engineering, glycerol kinase knock-out} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • PubMed
  • PubMed Central
  • Scopus
  • Google Scholar
  • DOAJ
  • CrossRef
  • Chemical Abstracts Service (CAS)
  • Science Citation Index Expanded


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