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Pregled bibliografske jedinice broj: 868854

Structural characterization of purine nucleoside phosphorylase from human pathogen Helicobacter pylori


Štefanić, Zoran; Mikleušević, Goran; Luić, Marija; Bzowska, Agnieszka; Leščić Ašler, Ivana
Structural characterization of purine nucleoside phosphorylase from human pathogen Helicobacter pylori // International journal of biological macromolecules, 101 (2017), 518-526 doi:10.1016/j.ijbiomac.2017.03.101 (međunarodna recenzija, članak, znanstveni)


Naslov
Structural characterization of purine nucleoside phosphorylase from human pathogen Helicobacter pylori

Autori
Štefanić, Zoran ; Mikleušević, Goran ; Luić, Marija ; Bzowska, Agnieszka ; Leščić Ašler, Ivana

Izvornik
International journal of biological macromolecules (0141-8130) 101 (2017); 518-526

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Helicobacter pylori ; Purine nucleoside phosphorylase ; Purification ; Stability ; Substrate specificity ; Crystal structure

Sažetak
Microaerophilic bacterium Helicobacer pylori is a well known human pathogen involved in the devel-opment of many diseases. Due to the evergrowing infection rate and increase of H. pylori antibioticresistence, it is of utmost importance to find a new way to attack and eradicate H. pylori. The purinemetabolism in H. pylori is solely dependant on the salvage pathway and one of the key enzymes in thispathway is purine nucleoside phosphorylase (PNP). In this timely context, we report here the basic bio-chemical and structural characterization of recombinant PNP from the H. pylori clinical isolate expressedin Escherichia coli. Structure of H. pylori PNP is typical for high molecular mass PNPs. However, its activitytowards adenosine is very low, thus resembling more that of low molecular mass PNPs. Understandingthe molecular mechanism of this key enzyme may lead to the development of new drug strategies andhelp in the eradication of H. pylori.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekt / tema
HRZZ-IP-2013-11-7423 - Enzimi purinskog reciklirajućeg ciklusa iz Helicobacter pylori i Escherichie coli (Marija Luić, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • EMBASE (Excerpta Medica)
  • FSTA: Food Science and Technology Abstracts
  • MEDLINE


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