Napredna pretraga

Pregled bibliografske jedinice broj: 868130

Flow Cytometer Monitoring of Bnip3- and Bnip3L/Nix- Dependent Mitophagy


Šprung, Matilda; Dikić, Ivan; Novak, Ivana
Flow Cytometer Monitoring of Bnip3- and Bnip3L/Nix- Dependent Mitophagy // Methods in molecular biology, 1759 (2018), 105-110 doi:10.1007/7651_2017_14 (međunarodna recenzija, članak, znanstveni)


Naslov
Flow Cytometer Monitoring of Bnip3- and Bnip3L/Nix- Dependent Mitophagy

Autori
Šprung, Matilda ; Dikić, Ivan ; Novak, Ivana

Izvornik
Methods in molecular biology (1940-6029) 1759 (2018); 105-110

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bnip3L/Nix ; Flow cytometry ; MitoTracker ; Mitophagy ; Mitophagy receptor

Sažetak
Mitochondria are organelles with numerous vital roles in cellular metabolism. Impaired or damaged mitochondria are degraded in autophagolysosomes in a process known as mitophagy. Given the fundamental role of mitophagy in maintenance of cellular homeostasis, methods and techniques with which to study this process are constantly evolving and emerging. So far, mitophagy flux was mostly monitored using fluorescently labeled LC3 protein on autophagosomal membrane and any of the labeled outer mitochondrial membrane proteins. However, this method is labor intensive, time consuming, and difficult to quantitatively validate due to the rapid mitochondrial turnover. Here, we describe a flow cytometry as a novel and promising quantitative method to monitor Bnip3- and Bnip3L/Nix-mediated mitophagy.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

Napomena
DOI:10.1007/7651_2017_14



POVEZANOST RADA


Projekt / tema
HRZZ-UIP-2013-11-5246 - Uloga receptora autofagije u selektivnom uklanjanju mitohondrija (Ivana Nakir, )

Ustanove
Prirodoslovno-matematički fakultet, Split,
Medicinski fakultet, Split

Časopis indeksira:


  • MEDLINE


Citati