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Pregled bibliografske jedinice broj: 867278

When screening for Down syndrome do we think about the preanalytics?


Krtanjek, Sanja; Petek Tarnik, Iva; Marijančević, Domagoj; Bokulić, Adriana; Bukovec Megla, Željka
When screening for Down syndrome do we think about the preanalytics? // 4th EFLM-BD European Conference on Preanalytical Phase Amsterdam (NL), 24–25 March 2017
Amsterdam, Nizozemska, 2017. (poster, međunarodna recenzija, sažetak, stručni)


CROSBI ID: 867278 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
When screening for Down syndrome do we think about the preanalytics?

Autori
Krtanjek, Sanja ; Petek Tarnik, Iva ; Marijančević, Domagoj ; Bokulić, Adriana ; Bukovec Megla, Željka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Skup
4th EFLM-BD European Conference on Preanalytical Phase Amsterdam (NL), 24–25 March 2017

Mjesto i datum
Amsterdam, Nizozemska, 24-25 ožujak 2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
free βhCG, PAPP-A, stability, room temperature

Sažetak
Background: On national level, only several specialized laboratories provide screening for Down syndrome. Our first aim was to confirm manufacturer claims that stability of free βhCG ( free beta-subunit of human chorionic gonadotropin ) and PAPP-A (pregnancy associated plasma protein A) in serum at room temperature is 8h. Requirements for sending samples by delivery service should depend on analyte stability. Most samples are being delivered within 24 or 48h. Our second aim was to investigate stability of free βhCG and PAPP-A in serum at room temperature within 24h and 48h. Materials and Method: Samples were collected from 30 women who came for Down syndrome screening in tubes with clot activator. Free βhCG and PAPP-A were measured (Roche Cobas e601) in primary samples: initially (I) and after 8h (II) and also in aliquoted samples: after 24h (III) and after 48h (IV) (all samples were stored at room temperature). Data were not normally distributed (Shapiro Wilk test) and were expressed as median (inter-quartile range). Differences between measurements were examined by Wilcoxon test. Results: Free βhCG median concentration in initially measurement was 51, 75 ng/mL (24, 17-70, 90), after 8h 53, 45 ng/mL (25, 58-74, 69), after 24h 53, 81 ng/mL ( 25, 30-75, 53) and after 48h 53, 52 ng/mL ( 27, 36-81, 35). PAPP-A median concentration in initially measurement was 3495 mUI/L (2108-6267), after 8h 3562 mUI/L (2100-6378), after 24h 3712 mUI/L (2195-6484) and after 48h 3928 mUI/L (2307-6275). Wilcoxon test showed statistically significant difference in free βhCG and PAPP-A concentration between (I) (II) (p<0, 001), (I) (III) (p<0, 001), (I) (IV) (p<0, 001). Conclusion: This study revealed that manufacturer claims about free βhCG and PAPP-A stability at room temperature within 8h are not confirmed. Also, free βhCG and PAPP-A concentrations increase after 24h and 48h at room temperature. Therefore, delivery protocol should be revised according to the investigated stability.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti



POVEZANOST RADA


Ustanove:
KBC "Sestre Milosrdnice"


Citiraj ovu publikaciju:

Krtanjek, Sanja; Petek Tarnik, Iva; Marijančević, Domagoj; Bokulić, Adriana; Bukovec Megla, Željka
When screening for Down syndrome do we think about the preanalytics? // 4th EFLM-BD European Conference on Preanalytical Phase Amsterdam (NL), 24–25 March 2017
Amsterdam, Nizozemska, 2017. (poster, međunarodna recenzija, sažetak, stručni)
Krtanjek, S., Petek Tarnik, I., Marijančević, D., Bokulić, A. & Bukovec Megla, Ž. (2017) When screening for Down syndrome do we think about the preanalytics?. U: 4th EFLM-BD European Conference on Preanalytical Phase Amsterdam (NL), 24–25 March 2017.
@article{article, author = {Krtanjek, Sanja and Petek Tarnik, Iva and Marijan\v{c}evi\'{c}, Domagoj and Bokuli\'{c}, Adriana and Bukovec Megla, \v{Z}eljka}, year = {2017}, keywords = {free βhCG, PAPP-A, stability, room temperature}, title = {When screening for Down syndrome do we think about the preanalytics?}, keyword = {free βhCG, PAPP-A, stability, room temperature}, publisherplace = {Amsterdam, Nizozemska} }
@article{article, author = {Krtanjek, Sanja and Petek Tarnik, Iva and Marijan\v{c}evi\'{c}, Domagoj and Bokuli\'{c}, Adriana and Bukovec Megla, \v{Z}eljka}, year = {2017}, keywords = {free βhCG, PAPP-A, stability, room temperature}, title = {When screening for Down syndrome do we think about the preanalytics?}, keyword = {free βhCG, PAPP-A, stability, room temperature}, publisherplace = {Amsterdam, Nizozemska} }




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