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Pregled bibliografske jedinice broj: 866652

Horseradish esterases: detection, purification and identification


Leščić Ašler, Ivana; Peharec Štefanić, Petra; Balen, Biljana; Allmaier, Günter; Marchetti- Deschmann, Martina; Kojić-Prodić, Biserka
Horseradish esterases: detection, purification and identification // Plant cell, tissue and organ culture, 130 (2017), 1; 13-24 doi:10.1007/s11240-017-1200-0 (međunarodna recenzija, članak, znanstveni)


Naslov
Horseradish esterases: detection, purification and identification

Autori
Leščić Ašler, Ivana ; Peharec Štefanić, Petra ; Balen, Biljana ; Allmaier, Günter ; Marchetti- Deschmann, Martina ; Kojić-Prodić, Biserka

Izvornik
Plant cell, tissue and organ culture (0167-6857) 130 (2017), 1; 13-24

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Armoracia lapathifolia ; Protein identification ; Protein purification ; Electrophoresis ; Mass spectrometry ; Esterase

Sažetak
Our goal is to characterize esterases from horseradish tissues and assign their physiological roles. In the present study we focused on isolation, purification and identification of esterases from different horseradish tissues: plantlets and two tumor tissue lines. Horizontal IEF system enabled separation of six esterase isoforms with quite different pI values as well as with pronounced differences in expression levels among analyzed tissues. Esterases were extracted, fractionated by means of cation exchange chromatography, and analyzed by planar gel electrophoresis (SDS– PAGE) and isoelectrical focusing (IEF), UV/Vis spectroscopy, MALDI mass spectrometry (MS) and MALDI-MS/MS. Several chromatographic strategies were applied for esterase purification and characterization. Two subsequent cation exchange chromatographic steps based on SP-Sepharose FF material, followed by in-solution digestion combined with MALDI-MS and MS/MS proved to be the best strategy for identification of two esterase proteins, namely Pectinesterase/pectinesterase inhibitor 18 and GDSL esterase/lipase ESM1.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija



POVEZANOST RADA


Ustanove
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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