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Pregled bibliografske jedinice broj: 863993

Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan


Grbavac, Antonija; Čanak, Iva; Stuparević, Igor; Teparić, Renata; Mrša, Vladimir
Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan // Biochimica et biophysica acta-molecular cell research, 1864 (2017), 3; 507-515 doi:10.1016/j.bbamcr.2016.12.009 (međunarodna recenzija, članak, znanstveni)


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Naslov
Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan

Autori
Grbavac, Antonija ; Čanak, Iva ; Stuparević, Igor ; Teparić, Renata ; Mrša, Vladimir

Izvornik
Biochimica et biophysica acta-molecular cell research (0167-4889) 1864 (2017), 3; 507-515

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cell wall proteins ; Kex2 ; Proteolytic processing ; Scw4 ; Yapsins ; Yeast cell wall

Sažetak
Yeast cell wall contains a number of proteins that are either non-covalently (Scw-proteins), or covalently (Ccw-proteins) bound to β-1, 3-glucan, the latter either through GPI-anchors and β-1, 6-glucan, or by alkali labile ester linkages between γ-carboxyl groups of glutamic acid and hydroxyl groups of glucoses (Pir-proteins). It was shown that a part of Scw4, previously identified among the non-covalently bound cell wall proteins, was covalently attached to wall polysaccharides by a so far unknown alkali sensitive linkage. Thus Scw4 could be released from cell walls by treatments with hot SDS, mild alkali, or β-1, 3-glucanases, respectively. It was further shown that non-covalently bound Scw4 (SDS released) underwent the Kex2 proteolytic processing. In this paper it was demonstrated that Scw4 was also processed by yapsins at a position 9 amino acids downstream of the Kex2 cleavage site. Scw4 cleaved at the yapsin site had a markedly lower potential for covalent attachment to glucan. The overproduction of the fully processed form of Scw4 lead to high mortality, particularly in the stationary phase of growth, and to markedly increased cell size. On the other hand, the overproduction of Scw4 processed only by Kex2 or not processed at all had no apparent change in mortality indicating that only the smallest, completely mature form of Scw4 had the activity leading to observed phenotype changes.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2014-09-2837 - Molekularni mehanizmi ugradnje homolognih i heterolognih proteina u staničnoj stijenci kvasca i njhova primjena (CEWAPROT) (Mrša, Vladimir, HRZZ - 2014-09) ( POIROT)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com dx.doi.org

Citiraj ovu publikaciju:

Grbavac, Antonija; Čanak, Iva; Stuparević, Igor; Teparić, Renata; Mrša, Vladimir
Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan // Biochimica et biophysica acta-molecular cell research, 1864 (2017), 3; 507-515 doi:10.1016/j.bbamcr.2016.12.009 (međunarodna recenzija, članak, znanstveni)
Grbavac, A., Čanak, I., Stuparević, I., Teparić, R. & Mrša, V. (2017) Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan. Biochimica et biophysica acta-molecular cell research, 1864 (3), 507-515 doi:10.1016/j.bbamcr.2016.12.009.
@article{article, year = {2017}, pages = {507-515}, DOI = {10.1016/j.bbamcr.2016.12.009}, keywords = {Cell wall proteins, Kex2, Proteolytic processing, Scw4, Yapsins, Yeast cell wall}, journal = {Biochimica et biophysica acta-molecular cell research}, doi = {10.1016/j.bbamcr.2016.12.009}, volume = {1864}, number = {3}, issn = {0167-4889}, title = {Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan}, keyword = {Cell wall proteins, Kex2, Proteolytic processing, Scw4, Yapsins, Yeast cell wall} }
@article{article, year = {2017}, pages = {507-515}, DOI = {10.1016/j.bbamcr.2016.12.009}, keywords = {Cell wall proteins, Kex2, Proteolytic processing, Scw4, Yapsins, Yeast cell wall}, journal = {Biochimica et biophysica acta-molecular cell research}, doi = {10.1016/j.bbamcr.2016.12.009}, volume = {1864}, number = {3}, issn = {0167-4889}, title = {Proteolytic processing of the Saccharomyces cerevisiae cell wall protein Scw4 regulates its activity and influences its covalent binding to glucan}, keyword = {Cell wall proteins, Kex2, Proteolytic processing, Scw4, Yapsins, Yeast cell wall} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Citati:





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