Cellular hydrogels based on pH-responsive chitosan- hydroxyapatite system (CROSBI ID 236943)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Rogina, Anamarija ; Ressler, Antonia ; Matić, Igor ; Gallego Ferrer, Gloria ; Marijanović, Inga ; Ivanković, Marica ; Ivanković, Hrvoje
engleski
Cellular hydrogels based on pH-responsive chitosan- hydroxyapatite system
The development of bioactive injectable system as cell carrier with minimal impact on viability of encapsulated cells represents a great challenge. In the present work, we propose a new pH- responsive chitosan-hydroxyapatite-based hydrogel with sodium bicarbonate (NaHCO3) as the gelling agent. The in situ synthesis of hydroxyapatite phase has resulted in stable composite suspension and final homogeneous hydrogel. The application of sodium bicarbonate has allowed non-cytotoxic fast gelation of chitosan-hydroxyapatite within 4 minutes, and without excess of sodium ions concentration. Rheological properties of crosslinked hydrogel have demonstrated possible behaviour as ‘strong physical hydrogel’. The live dead staining has confirmed good viability and dispersion, as well as proliferation of encapsulated cells by the culture time. Presented preliminary results show good potential of chitosan-hydroxyapatite/NaHCO3 as a cell carrier, whose impact on the cell differentiation need to be confirmed by encapsulation of other cell phenotypes.
chitosan ; hydroxyapatite ; pH-responsive hydrogel ; rheology ; cell encapsulation
Glavni autori: Anamarija Rogina i Marica Ivanković
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Podaci o izdanju
166
2017.
173-182
objavljeno
0144-8617
1879-1344
10.1016/j.carbpol.2017.02.105
Povezanost rada
Biologija, Kemija, Kemijsko inženjerstvo, Temeljne tehničke znanosti