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Pregled bibliografske jedinice broj: 863451

Mobile introns shape the genetic diversity of host genes

Repar, Jelena; Warnecke, Tobias
Mobile introns shape the genetic diversity of host genes // Genetics, 205 (2017), 4; 1641-1648 doi:10.1534/genetics.116.199059 (međunarodna recenzija, članak, znanstveni)

Mobile introns shape the genetic diversity of host genes

Repar, Jelena ; Warnecke, Tobias

Genetics (0016-6731) 205 (2017), 4; 1641-1648

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Genetic diversity ; self-splicing introns ; mutagenic ; gene conversion ; homing endonuclease

Self-splicing introns populate several highly conserved protein-coding genes in fungal and plant mitochondria. In fungi, many of these introns have retained their ability to spread to intron-free target sites, often assisted by intron-encoded endonucleases that initiate the homing process. Here, leveraging population genomic data from Saccharomyces cerevisiae, Schizosaccharomyces pombe, and Lachancea kluyveri, we expose non-random patterns of genetic diversity in exons that border self-splicing introns. In particular, we show that, in all three species, the density of single nucleotide polymorphisms increases as one approaches a mobile intron. Through multiple lines of evidence we rule out relaxed purifying selection as the cause of uneven nucleotide diversity. Instead, our findings implicate intron mobility as a direct driver of host gene diversity. We discuss two mechanistic scenarios that are consistent with the data: either endonuclease activity and subsequent error-prone repair have left a mutational footprint on the insertion environment of mobile introns or non-random patterns of genetic diversity are caused by exonic co-conversion, which occurs when introns spread to empty target sites via homologous recombination. Importantly, however, we show that exonic co-conversion can only explain diversity gradients near intron-exon boundaries if the conversion template comes from outside the population. In other words, there must be pervasive and ongoing horizontal gene transfer of self-splicing introns into extant fungal populations.

Izvorni jezik

Znanstvena područja
Biologija, Računarstvo


Autor s matičnim brojem:
Jelena Repar, (290662)

Časopis indeksira:

  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus