engleski

Histomorphometric assessment of pentachlorophenol toxic effects on a digestive gland of the freshwater snail Holandriana holandrii (C. Pfeiffer, 1828)

Poznato je kako je zagađenje pesticidima sveprisutno u vodenim ekosustavima. U ovom radu analizirali smo toksične učinke natrijevog pentaklorofenolata (Na-PCP) na vrstu slatkovodnog puža Holandriana holandrii (C. Pfeiffer, 1828) s ciljem utvrđivanja histopatoloških i histomorfometrijskih promjena probavne žlijezde. Probavnu žlijezdu puževa sačinjavaju brojni razgranati kanalići građeni od probavnih i bazofilnih stanica. Jedinke puževa smo izložili toksinu, Na-PCP-u, u tri koncentracije (0, 56 mg/L, 1, 0 mg/L, 5, 6 mg/L) te smo usporedili histološke promjene između analiziranih koncentracija tijekom 96-satnog trajanja pokusa. Histopatološke promjene očitovale su se u vakuolizaciji stanica, nakupljanju eksudata unutar lumena tubula, ljuštenju stanica tubula i destrukciji tubula. Histomorfometrijske vrijednosti veličine lumena, odnosa površine stijenke i lumena tubula kao i eksudata, ukazuju na statističku značajnu razliku kako između testiranih koncentracija tako i tijekom istraživanog akutnog razdoblja trovanja. Smatramo da histomorfometrijske metode mogu biti korisne u analizi štetnih učinaka toksičnih spojeva na slatkovodne organizme. Pesticid pollutants are recorded in varying levels in all aquatic ecosystems. In this study we analysed toxic effects of sodiumpentachlorophenate (Na-PCP) on freshwater snail, Holandriana holandrii (C. Pfeiffer, 1828) with the aim to determine histopathological and histomorphometrical alterations in digestive gland. The digestive gland of snails consists of numerous branched tubules overlaid with digestive and basophilic cells. We exposed snail specimens to toxic substance, Na-PCP, in three concentrations (0.56 mg/L, 1.0 mg/L and 5.6 mg/L) and compared histological alterations among analysed concentrations during a 96-hour test. The following histopathological changes were observed: cell vacuolization, exudate effusion, shedding of tubular cells and disorganization of tubules. The results of histomorphometric analysis indicated that all measured changes, i.e., ratio between area of tubule wall and lumen as well as exudate area, increased during the period of acute toxicity and significantly differed among concentrations and time. We consider that histomorphometry can serve as an useful tool in recognizing harmful effects of toxic compounds on freshwater organisms.

PCP, digestive and basophil cells, tubul, exudat

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