engleski

The use of optical spectroscopy in determining embryo viability in assisted reproductive technology

Embryo selection in assisted reproductive technology remains relatively imprecise to this day. Multiple embryo transfers increase the likelihood of implantation and clinical pregnancy, but they also increase the risk of multiple pregnancy, dangerous to both mother and foetus. If there was enough confi dence in choosing a single, most viable embryo, single embryo transfer would become the method of choice more often. While an ideal method to determine embryo viability still doesn’t exist, studying the metabolome of a spent culture medium shows some promise. Optical spectroscopy techniques are non-invasive and provide quick insight into metabolic profi les of spent culture media. Several studies show that near- infrared, Fourier transformation infrared and Raman spectroscopy could determine embryo’s viability and predict its implantation potential with increased accuracy compared to morphology assessment conducted by man, although some studies show opposite results. Various obstacles were noted in studies published so far, including inconsistent results because the algorithms for determining embryo viability and the commercial instruments were not synchronized. Although optical spectroscopy in studying the spent culture media to determine embryo viability prior to transfer shows promise, further and larger studies are needed to assess their potential.

medically assisted reproduction, embrio, viability, optical spectroscopy

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