engleski

Quercetin attenuates n-Hexyl salicylate or Di-n-Propyl Disulfide - induced skin irritation: oxidative stress and inflammation

Reactive oxygen species (ROS) play a role in a numbered of chronic diseases including psoriasis. Psoriasis is a chronic inflammatory, proliferative skin disease characterized by hyperkeratosis, dermal inflammatory infiltrate and increased angiogenesis. The current study examined the possible positive effects of quercetin on animal model psoriasis, induced by the Hexyl salicylate (HXS) or Di-n-Propyl Disulfide irritant (PPD) and possibility to assess usefulness of thermography in psoriatic lesion regression. We monitored the inflammation process by histopathological assessment of skin, thermographic scanning, total number of inflammatory cells in peritoneal cavity, differential analysis of cells in peritoneal cavity, macrophage spreading index, hematological and biochemical parameters, frequencies of micronucleated reticulocytes, lipid peroxidation and glutathione assay in skin, liver and kidney. Topically applied quercetin with HXS or PPD may improve psoriatic-like skin lesions by suppressing number of inflammatory cells in skin, functional activity of macrophages, and ROS production. In addition, total number of inflammatory cells in peritoneal cavity, macrophage spreading index, lipid peroxidation in liver and kidney, and enzymatic activity of ALP and AST were reduced in psoriatic mice treated with quercetin. Taken together, these results support the quercetin as an anti-inflammatory and antioxidative agent and open up new possibilities for its use in skin disorders without causing any toxic effect. Thermal imaging was realistic, and can be applicable in examining of the inflammatory process in psoriasis and in evaluating the effectiveness of quercetin and other tested substances.

mouse models of psoriasis, skin lesions, thermography, quercetin

Reactive oxygen species (ROS) play a role in a numbered of chronic diseases including psoriasis. Psoriasis is a chronic inflammatory, proliferative skin disease characterized by hyperkeratosis, dermal inflammatory infiltrate and increased angiogenesis. The current study examined the possible positive effects of quercetin on animal model psoriasis, induced by the Hexyl salicylate (HXS) or Di-n-Propyl Disulfide irritant (PPD) and possibility to assess usefulness of thermography in psoriatic lesion regression. We monitored the inflammation process by histopathological assessment of skin, thermographic scanning, total number of inflammatory cells in peritoneal cavity, differential analysis of cells in peritoneal cavity, macrophage spreading index, hematological and biochemical parameters, frequencies of micronucleated reticulocytes, lipid peroxidation and glutathione assay in skin, liver and kidney. Topically applied quercetin with HXS or PPD may improve psoriatic-like skin lesions by suppressing number of inflammatory cells in skin, functional activity of macrophages, and ROS production. In addition, total number of inflammatory cells in peritoneal cavity, macrophage spreading index,