Non-toxic fluorescent phosphonium probes to detect mitochondrial potential (CROSBI ID 236448)
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Podaci o odgovornosti
Šarić, Ana ; Crnolatac, Ivo ; Bouillaud, Frédéric ; Sobocanec, Sandra ; Mikecin, Ana- Matea ; Mačak Šafranko, Željka ; Deligeorgiev, Todor ; Piantanida, Ivo ; Balog, Tihomir ; Petit, Patrice
engleski
Non-toxic fluorescent phosphonium probes to detect mitochondrial potential
We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage, and probe binding. Here presented probes are characterized by highly efficient cellular uptake and specific accumulation in mitochondria. Fluorescent detection of the probes was accomplished using flow cytometry and confocal microscopy imaging of yeast and mammalian cells. Toxicity analysis (impedimetry - xCELLigence for the cellular proliferation and Seahorse technology for respiratory properties) confirms that these dyes exhibit no toxicity on mitochondrial or cellular functioning even for long time incubation. The excellent chemical and photophysical stability of the dyes make them promising leads toward improved fluorescent probes. Therefore, the probes described here offer to circumvent the problems associated with existing probes limitations.
Fluorescence ; Mitochondria ; Membrane potential ; Probe ; Phophonium
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Podaci o izdanju
5 (1)
2017.
015007
13
objavljeno
2050-6120
10.1088/2050-6120/aa5e64