engleski

Maternal high-fat diet during pregnancy and lactation and effects on male offspring weight and organ development

Aim: Obesity, as global epidemic, induces many different disorders, just to mention metabolic syndrome. Several studies have tried to explain the correlation between maternal nutrition and consequences in metabolic profile of offspring, having in mind hypothesis of a "thrifty phenotype" as an adaptive mechanism preparing the organism to its likely adult environment. In this study, we investigated the effects of maternal high-fat diet prior and during pregnancy and lactatiton on weight, organ development and laboratory findings in male offspring. Methods: Ten female Sprague Dawley rats, 9 weeks old, were randomly divided in two groups. One group was fed with high-fat diet (HFD group), the other with standard laboratory chow (CD group). After pregnancy and lactation male offspring were also randomly divided in two groups each- HFD and CD. At 12 weeks of age the offspring were sacrificed and blood and tissue samples collected. From blood samples total cholesterole, trygliceride and glucose level, IL-6 and TNF-alpha were measured. Organs weight: heart, liver, testis, kidney, spleen, thymus and lungs were measured. Results: We observed an increase in body and organ weight in all organs except thymus in offspring fed high-fat diet compared to CD-CD group. No significant difference in organ weight between CD-HFD and HFD-HFD group was observed. There was no significant difference in tryglicerides, glucose, IL-6 and TNF-alpha levels between the groups where dams and/or offspring were fed with high-fat diet. Total cholesterole level in serum was increased in aforementioned groups. Conclusion: Our results show that high-fat diet affects organ development.

hgh-fat diet, offspring, weight, organ development

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