engleski

Influence of changes in nutritional protocol between generations on vaginal smear characteristics

Aim: Changes in feeding protocol between generations have influence on metabolic functioning, especially on female reproductive physiology. The aim of the study was to determinate impact of mothers` nutrition and nutrition of offspring on characteristics of vaginal smears and lipid levels in offspring. Methods: Nine weeks old female Sprague Dawley rats (n=10) were randomly divided in two groups: high fat diet (HFD, n=5) group which was fed during 6 weeks with high content of saturated fatty acid food and control diet (CD, n=5) group which was given standard laboratory chow during the same period. Offspring from both groups were randomly divided in two subgroups after coupling and lactation period, subsequently there were four groups of offspring with different feeding protocol: CD offspring of CD mothers (n=6), HFD offspring of CD mothers (n=6), CD offspring of HFD mothers (n=6), HFD offspring of HFD mothers (n=6). All rats were weight and blood was collected at the age of 18 weeks, when vaginal smears collection was started during 15 days period. Materials were stained following May-Grünwald-Giemsa staining protocol and observed under light microscope. Results: Groups in which the nutrition protocol between mothers and offspring was changed showed significant changes in characteristics of vaginal smears, whereas the same nutrition protocol between mothers and offspring resulted in normal characteristics of vaginal smears. Significant higher triglyceride level was in HFD-HFD and cholesterol level in CD-HFD group. Conclusion: Maternal HFD consumption predisposes offspring to increased risk of developing metabolic abnormalities and estrus disorders.

hgh-fat diet, offspring, reproduction

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