Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 859981

Effects of Celastrol on Bv-2 Microglial Cells Exposed to in Vitro Hypoxia


Rački, Valentino; Marcelić, Marina; Mršić-Pelčić, Jasenka; Kučić, Natalia
Effects of Celastrol on Bv-2 Microglial Cells Exposed to in Vitro Hypoxia // Clinical Therapeutics
Opatija, Hrvatska, 2016. str. e21-e21 (poster, nije recenziran, sažetak, ostalo)


CROSBI ID: 859981 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Effects of Celastrol on Bv-2 Microglial Cells Exposed to in Vitro Hypoxia

Autori
Rački, Valentino ; Marcelić, Marina ; Mršić-Pelčić, Jasenka ; Kučić, Natalia

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Clinical Therapeutics / - , 2016, E21-e21

Skup
EACPT Focus Meeting 2016.

Mjesto i datum
Opatija, Hrvatska, 06-09.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
microglia ; hypoxia ; celastrol

Sažetak
Introduction: Microglia are a subset of macrophages permanently present in the central nervous system. Apart from being the key effector of the innate immune response, microglia play a major role in the maintenance of homeostasis. They represent the first line of defense in the central nervous system and as such are affected by all changes in their environment. Microglia respond to hypoxia with acute inflammation, followed by a tendency for an anti-inflammatory switch during the late post-hypoxia period. Celastrol, a naturally occurring quinone methide triterpene derived from the Thunder of God Vine is a pleiotropic compound showing anti-inflammatory, anti-tumor, anti-hypertensive and anti-diabetic activity in numerous cellular and in vivo models. The aim of our research was to determine the effects of celastrol on microglial cells exposed to in vitro hypoxic conditions. Materials and methods: We used murine microglial BV-2 cell line which is commonly used as a substitute for primary microglia. The cells were treated with 100nM of celastrol before or after hypoxia (<0.1% O2, 98% N2), which was induced in a hyperbaric chamber. We analyzed the expression of COX-2, HSP-70 and MHC-1, as respective markers of inflammation, cellular stress and immune response. Measurement of protein expression was performed with Western blot analysis. The measurement was performed in 5 time periods: 0h, 24h, 48h, 72h and 168h post- hypoxia. Results: Hypoxic cells that were not treated with celastrol exhibited increased expression of COX-2, HSP-70 and MHC-1. Treatment with celastrol, both before and after hypoxia, lowered the expression of mentioned markers in all time periods compared to the control. Conclusions: Our results indicate that celastrol acted as anti-inflammatory agent that effectively reduced cellular stress. In addition, celastrols effect on the expression of MHC-1, indicated its possible immunomodulatory potential. Further study is required as celastrol could be used as an anti- inflammatory agent due to the aforementioned effects.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove:
Medicinski fakultet, Rijeka

Citiraj ovu publikaciju

Rački, Valentino; Marcelić, Marina; Mršić-Pelčić, Jasenka; Kučić, Natalia
Effects of Celastrol on Bv-2 Microglial Cells Exposed to in Vitro Hypoxia // Clinical Therapeutics
Opatija, Hrvatska, 2016. str. e21-e21 (poster, nije recenziran, sažetak, ostalo)
Rački, V., Marcelić, M., Mršić-Pelčić, J. & Kučić, N. (2016) Effects of Celastrol on Bv-2 Microglial Cells Exposed to in Vitro Hypoxia. U: Clinical Therapeutics.
@article{article, year = {2016}, pages = {e21-e21}, keywords = {microglia, hypoxia, celastrol}, title = {Effects of Celastrol on Bv-2 Microglial Cells Exposed to in Vitro Hypoxia}, keyword = {microglia, hypoxia, celastrol}, publisherplace = {Opatija, Hrvatska} }
@article{article, year = {2016}, pages = {e21-e21}, keywords = {microglia, hypoxia, celastrol}, title = {Effects of Celastrol on Bv-2 Microglial Cells Exposed to in Vitro Hypoxia}, keyword = {microglia, hypoxia, celastrol}, publisherplace = {Opatija, Hrvatska} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
  • Scopus
  • MEDLINE





Contrast
Increase Font
Decrease Font
Dyslexic Font